Vicia faba SV channel VfTPC1 is a hyperexcitable variant of plant vacuole two pore channels
Please always quote using this URN: urn:nbn:de:bvb:20-opus-350264
- To fire action-potential-like electrical signals, the vacuole membrane requires the two-pore channel TPC1, formerly called SV channel. The TPC1/SV channel functions as a depolarization-stimulated, non-selective cation channel that is inhibited by luminal Ca\(^{2+}\). In our search for species-dependent functional TPC1 channel variants with different luminal Ca\(^{2+}\) sensitivity, we found in total three acidic residues present in Ca\(^{2+}\) sensor sites 2 and 3 of the Ca\(^{2+}\)-sensitive AtTPC1 channel from Arabidopsis thaliana that wereTo fire action-potential-like electrical signals, the vacuole membrane requires the two-pore channel TPC1, formerly called SV channel. The TPC1/SV channel functions as a depolarization-stimulated, non-selective cation channel that is inhibited by luminal Ca\(^{2+}\). In our search for species-dependent functional TPC1 channel variants with different luminal Ca\(^{2+}\) sensitivity, we found in total three acidic residues present in Ca\(^{2+}\) sensor sites 2 and 3 of the Ca\(^{2+}\)-sensitive AtTPC1 channel from Arabidopsis thaliana that were neutral in its Vicia faba ortholog and also in those of many other Fabaceae. When expressed in the Arabidopsis AtTPC1-loss-of-function background, wild-type VfTPC1 was hypersensitive to vacuole depolarization and only weakly sensitive to blocking luminal Ca\(^{2+}\). When AtTPC1 was mutated for these VfTPC1-homologous polymorphic residues, two neutral substitutions in Ca\(^{2+}\) sensor site 3 alone were already sufficient for the Arabidopsis At-VfTPC1 channel mutant to gain VfTPC1-like voltage and luminal Ca\(^{2+}\) sensitivity that together rendered vacuoles hyperexcitable. Thus, natural TPC1 channel variants exist in plant families which may fine-tune vacuole excitability and adapt it to environmental settings of the particular ecological niche.…
Author: | Jinping LuORCiD, Ingo Dreyer, Miles Sasha Dickinson, Sabine PanzerORCiD, Dawid JaślanORCiD, Carlos Navarro-Retamal, Dietmar GeigerORCiD, Ulrich TerpitzORCiD, Dirk BeckerORCiD, Robert M. Stroud, Irene MartenORCiD, Rainer HedrichORCiD |
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URN: | urn:nbn:de:bvb:20-opus-350264 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften |
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften | |
Language: | English |
Parent Title (English): | eLife |
Year of Completion: | 2023 |
Volume: | 12 |
Article Number: | e86384 |
Source: | eLife (2023) 12:e86384. DOI: 10.7554/eLife.86384 |
DOI: | https://doi.org/10.7554/eLife.86384 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik) |
Tag: | A. thaliana; Brassicaceae; Fabaceae; pore; potassium channel; vacuolar calcium sensor; voltage gating |
Release Date: | 2024/04/24 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |