Immunhistochemische Darstellung von spannungsabhängigen Calciumkanälen an peripheren afferenten Nervenfasern im Kniegelenk der Maus
Demonstration of voltage dependent calcium channels on calcitonin gene- related peptide-immunoreactive nerve fibres in the mouse knee joint
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- The afferent function (excitability) and efferent function (release of neuropeptides) of primary afferent nerve fibres is based on Ca2+-influx. Aim of the present study was to examine the presence of L-and N-type Ca2+-channels at sensory nerve fibres in the mouse knee joint capsule. Specific fluorescent labelled channel blocker and antisera against these channel subtypes were combined with an immunhistochemical staining for calcitonin gene-related peptide (CGRP), a neuropeptide that is wildly distributed in primary afferents. There was nearlyThe afferent function (excitability) and efferent function (release of neuropeptides) of primary afferent nerve fibres is based on Ca2+-influx. Aim of the present study was to examine the presence of L-and N-type Ca2+-channels at sensory nerve fibres in the mouse knee joint capsule. Specific fluorescent labelled channel blocker and antisera against these channel subtypes were combined with an immunhistochemical staining for calcitonin gene-related peptide (CGRP), a neuropeptide that is wildly distributed in primary afferents. There was nearly complete colocalisation of calcitonin gene-related peptide immunoreactivity and the binding of conotoxin GVIA or BODIPY- verapamil demonstrating the presence of N-type and L-type Ca2+-channels, respectively. These data were further confirmed by identical results obtained after an immunohistochemical demonstration of the two channel subtypes at the peptidergic nerve fibres.…
Autor(en): | Carsten Leipold-Büttner |
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URN: | urn:nbn:de:bvb:20-opus-17894 |
Dokumentart: | Dissertation |
Titelverleihende Fakultät: | Universität Würzburg, Medizinische Fakultät |
Institute der Universität: | Medizinische Fakultät / Physiologisches Institut |
Datum der Abschlussprüfung: | 01.05.2006 |
Sprache der Veröffentlichung: | Deutsch |
Erscheinungsjahr: | 2005 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | Afferenzen; CGRP; Calciumkanäle; Nozizeption CGRP; calcium channel; nociception; primary afferent |
Datum der Freischaltung: | 19.05.2006 |
Betreuer: | Priv.-Doz. Dr. Bernd Heppelmann |