The influence of MHC class II antigen blockade by perfusion with a monoclonal antibody on rat renal graft survival
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- To decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class li monoclonal antibody (mAb ). How effectively this procedure blocked dass li-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions were applied for graft pretreatment before transplantation. This procedure prolonged graft survival, though not satisfactorily from the biological point ofview (9.6 ± 0.8 versus 7.7 ± 0.5 daysTo decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class li monoclonal antibody (mAb ). How effectively this procedure blocked dass li-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions were applied for graft pretreatment before transplantation. This procedure prolonged graft survival, though not satisfactorily from the biological point ofview (9.6 ± 0.8 versus 7.7 ± 0.5 days in the control group; P < 0.02). The dendritic cells were not killed but blocked. Several hours after transplantation, the mAb dissociated from these dass li-positive cells. It was also shown that donor cells migrate into the recipient's spieen early after transplantation. The number of these cells was smaller when the transplanted organ was perfused with the mAb. Further studies are suggested to deplete the graft of donor dendritic cells more adequately. They should also combine graft perfusion with antidass II mAb and recipient immunosuppression at reduced doses.…
Autor(en): | Maciej Krzymanski, Ana M. Waaga, Karin Ulrichs, Aadam Deja, Andrzej Oko, Thomas Rommel, Wolfgang Müller-Ruchholtz |
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URN: | urn:nbn:de:bvb:20-opus-64431 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I) |
Sprache der Veröffentlichung: | Englisch |
Erscheinungsjahr: | 1991 |
Originalveröffentlichung / Quelle: | In: Transplant international (1991) Sep.4, 3, 180-5. |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Normierte Schlagworte (GND): | Chirurgie |
Freie Schlagwort(e): | Class II antigen blockade; Monoclonal antibody; dass II antigen blockade; rat; renal transplantation |
Datum der Freischaltung: | 17.08.2012 |
Lizenz (Deutsch): | Deutsches Urheberrecht |