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Characterization of Metastasis Formation and Virotherapy in the Human C33A Cervical Cancer Model
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-119674
- More than 90% of cancer mortalities are due to cancer that has metastasized. Therefore, it is crucial to intensify research on metastasis formation and therapy. Here, we describe for the first time the metastasizing ability of the human cervical cancer cell line C33A in athymic nude mice after subcutaneous implantation of tumor cells. In this model, we demonstrated a steady progression of lumbar and renal lymph node metastases during tumor development. Besides predominantly occurring lymphatic metastases, we visualized the formation ofMore than 90% of cancer mortalities are due to cancer that has metastasized. Therefore, it is crucial to intensify research on metastasis formation and therapy. Here, we describe for the first time the metastasizing ability of the human cervical cancer cell line C33A in athymic nude mice after subcutaneous implantation of tumor cells. In this model, we demonstrated a steady progression of lumbar and renal lymph node metastases during tumor development. Besides predominantly occurring lymphatic metastases, we visualized the formation of hematogenous metastases utilizing red fluorescent protein (RFP) expressing C33A-RFP cells. RFP positive cancer cells were found migrating in blood vessels and forming micrometastases in lungs of tumor-bearing mice. Next, we set out to analyze the influence of oncolytic virotherapy in the C33A-RFP model and demonstrated an efficient virus-mediated reduction of tumor size and metastatic burden. These results suggest the C33A-RFP cervical cancer model as a new platform to analyze cancer metastases as well as to test novel treatment options to combat metastases.…
Autor(en): | Ulrike Donat, Juliane Rother, Simon Schäfer, Michael Hess, Barbara Härtl, Christina Kober, Johanna Langbein-Laugwitz, Jochen Stritzker, Nanhai G. Chen, Richard J. Aguilar, Stephanie Weibel, Alandar A. Szalay |
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URN: | urn:nbn:de:bvb:20-opus-119674 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Rudolf-Virchow-Zentrum |
Fakultät für Chemie und Pharmazie / Lehrstuhl für Biochemie | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | PLoS ONE |
ISSN: | 1932-6203 |
Erscheinungsjahr: | 2014 |
Band / Jahrgang: | 9 |
Heft / Ausgabe: | 6 |
Seitenangabe: | e98533 |
Originalveröffentlichung / Quelle: | PLoS ONE 9(6): e98533. doi:10.1371/journal.pone.0098533 |
DOI: | https://doi.org/10.1371/journal.pone.0098533 |
PubMed-ID: | https://pubmed.ncbi.nlm.nih.gov/24887184 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 616 Krankheiten |
Freie Schlagwort(e): | cancer treatment; kidneys; lung and intrathoracic tumors; lymph nodes; metastasis; oncolytic viruses; renal cancer; secondary lung tumors |
Datum der Freischaltung: | 11.11.2015 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung |