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Doxycycline Inducible Melanogenic Vaccinia Virus as Theranostic Anti-Cancer Agent

Please always quote using this URN: urn:nbn:de:bvb:20-opus-124987
  • We reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouseWe reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouse models. Furthermore, it was confirmed that this novel vaccinia virus strain still facilitated signal enhancement as detected by MRI and optoacoustic tomography. At the same time we demonstrated an enhanced oncolytic potential compared to the constitutively melanin synthesizing rVACV system.show moreshow less

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Metadaten
Author: Lorenz Kirscher, Xosé Luis Deán-Ben, Miriam Scadeng, Angelika Zaremba, Qian Zhang, Christina Kober, Thomas Felix Fehm, Daniel Razansky, Vasilis Ntziachristos, Jochen Stritzker, Aladar A. Szalay
URN:urn:nbn:de:bvb:20-opus-124987
Document Type:Journal article
Faculties:Fakultät für Chemie und Pharmazie / Lehrstuhl für Biochemie
Language:English
Parent Title (English):Theranostics
Year of Completion:2015
Volume:5
Issue:10
Pagenumber:1045-1057
Source:Theranostics 2015; 5(10): 1045-1057. doi: 10.7150/thno.12533
DOI:https://doi.org/10.7150/thno.12533
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:molecular imaging; oncolysis; reporter gene; virotherapy
Release Date:2016/02/02
Collections:Open-Access-Publikationsfonds / Förderzeitraum 2015
Licence (German):License LogoCC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitung