The circadian clock is required for rhythmic lipid transport in Drosophila in interaction with diet and photic condition
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-349961
- Modern lifestyle is often at odds with endogenously driven rhythmicity, which can lead to circadian disruption and metabolic syndrome. One signature for circadian disruption is a reduced or altered metabolite cycling in the circulating tissue reflecting the current metabolic status. Drosophila is a well-established model in chronobiology, but day-time dependent variations of transport metabolites in the fly circulation are poorly characterized. Here, we sampled fly hemolymph throughout the day and analyzed diacylglycerols (DGs),Modern lifestyle is often at odds with endogenously driven rhythmicity, which can lead to circadian disruption and metabolic syndrome. One signature for circadian disruption is a reduced or altered metabolite cycling in the circulating tissue reflecting the current metabolic status. Drosophila is a well-established model in chronobiology, but day-time dependent variations of transport metabolites in the fly circulation are poorly characterized. Here, we sampled fly hemolymph throughout the day and analyzed diacylglycerols (DGs), phosphoethanolamines (PEs) and phosphocholines (PCs) using LC-MS. In wild-type flies kept on sugar-only medium under a light-dark cycle, all transport lipid species showed a synchronized bimodal oscillation pattern with maxima at the beginning and end of the light phase which were impaired in period01 clock mutants. In wild-type flies under constant dark conditions, the oscillation became monophasic with a maximum in the middle of the subjective day. In strong support of clock-driven oscillations, levels of the targeted lipids peaked once in the middle of the light phase under time-restricted feeding independent of the time of food intake. When wild-type flies were reared on full standard medium, the rhythmic alterations of hemolymph lipid levels were greatly attenuated. Our data suggest that the circadian clock aligns daily oscillations of DGs, PEs, and PCs in the hemolymph to the anabolic siesta phase, with a strong influence of light on phase and modality.…
Autor(en): | Kelechi M. Amatobi, Ayten Gizem Ozbek-UnalORCiD, Stefan Schäbler, Peter DeppischORCiD, Charlotte Helfrich-FörsterORCiD, Martin J. Mueller, Christian WegenerORCiD, Agnes FeketeORCiD |
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URN: | urn:nbn:de:bvb:20-opus-349961 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Theodor-Boveri-Institut für Biowissenschaften |
Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Journal of Lipid Research |
Erscheinungsjahr: | 2023 |
Band / Jahrgang: | 64 |
Heft / Ausgabe: | 10 |
Seitenangabe: | 100417 |
Originalveröffentlichung / Quelle: | Journal of Lipid Research (2023) 64:10, 100417. DOI: 10.1016/j.jlr.2023.100417 |
DOI: | https://doi.org/10.1016/j.jlr.2023.100417 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Freie Schlagwort(e): | circadian rhythm; feeding; hemolymph lipids; light-driven metabolism; lipidomics; locomotor activity |
Datum der Freischaltung: | 20.03.2024 |
Lizenz (Deutsch): | CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International |