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Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development

Please always quote using this URN: urn:nbn:de:bvb:20-opus-132626
  • Background: During early stages of brain development, secreted molecules, components of intracellular signaling pathways and transcriptional regulators act in positive and negative feed-back or feed-forward loops at the mid-hindbrain boundary. These genetic interactions are of central importance for the specification and subsequent development of the adjacent mid-and hindbrain. Much less, however, is known about the regulatory relationship and functional interaction of molecules that are expressed in the tectal anlage after tectal fateBackground: During early stages of brain development, secreted molecules, components of intracellular signaling pathways and transcriptional regulators act in positive and negative feed-back or feed-forward loops at the mid-hindbrain boundary. These genetic interactions are of central importance for the specification and subsequent development of the adjacent mid-and hindbrain. Much less, however, is known about the regulatory relationship and functional interaction of molecules that are expressed in the tectal anlage after tectal fate specification has taken place and tectal development has commenced. Results: Here, we provide experimental evidence for reciprocal regulation and subsequent cooperation of the paired-type transcription factors Pax3, Pax7 and the TALE-homeodomain protein Meis2 in the tectal anlage. Using in ovo electroporation of the mesencephalic vesicle of chick embryos we show that (i) Pax3 and Pax7 mutually regulate each other's expression in the mesencephalic vesicle, (ii) Meis2 acts downstream of Pax3/7 and requires balanced expression levels of both proteins, and (iii) Meis2 physically interacts with Pax3 and Pax7. These results extend our previous observation that Meis2 cooperates with Otx2 in tectal development to include Pax3 and Pax7 as Meis2 interacting proteins in the tectal anlage. Conclusion: The results described here suggest a model in which interdependent regulatory loops involving Pax3 and Pax7 in the dorsal mesencephalic vesicle modulate Meis2 expression. Physical interaction with Meis2 may then confer tectal specificity to a wide range of otherwise broadly expressed transcriptional regulators, including Otx2, Pax3 and Pax7.show moreshow less

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Metadaten
Author: Zsuzsa Agoston, Naixin Li, Anja Haslinger, Andrea Wizenmann, Dorothea Schulte
URN:urn:nbn:de:bvb:20-opus-132626
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):BMC Developmental Biology
Year of Completion:2012
Volume:12
Issue:10
Source:BMC Developmental Biology 2012, 12:10. doi:10.1186/1471-213X-12-10
DOI:https://doi.org/10.1186/1471-213X-12-10
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 573 Einzelne physiologische Systeme bei Tieren
Tag:dosage; drosophila embryo; expression; hindbrain boundary; induction; isthmic oragnizer; neural crest; quali-chick chimeras; sonic hedghog; transcription activation
Release Date:2017/01/12
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung