The Expandables: Cracking the Staphylococcal Cell Wall for Expansion Microscopy
Please always quote using this URN: urn:nbn:de:bvb:20-opus-232292
- Expansion Microscopy (ExM) is a novel tool improving the resolution of fluorescence microscopy by linking the sample into a hydrogel that gets physically expanded in water. Previously, we have used ExM to visualize the intracellular Gram-negative pathogens Chlamydia trachomatis, Simkania negevensis, and Neisseria gonorrhoeae. Gram-positive bacteria have a rigid and thick cell wall that impedes classic expansion strategies. Here we developed an approach, which included a series of enzymatic treatments resulting in isotropic 4× expansion of theExpansion Microscopy (ExM) is a novel tool improving the resolution of fluorescence microscopy by linking the sample into a hydrogel that gets physically expanded in water. Previously, we have used ExM to visualize the intracellular Gram-negative pathogens Chlamydia trachomatis, Simkania negevensis, and Neisseria gonorrhoeae. Gram-positive bacteria have a rigid and thick cell wall that impedes classic expansion strategies. Here we developed an approach, which included a series of enzymatic treatments resulting in isotropic 4× expansion of the Gram-positive pathogen Staphylococcus aureus. We further demonstrate the suitability of the technique for imaging of planktonic bacteria as well as endocytosed, intracellular bacteria at a spatial resolution of approximately 60 nm with conventional confocal laser scanning microscopy.…
Author: | Tobias C. Kunz, Marcel Rühling, Adriana Moldovan, Kerstin Paprotka, Vera Kozjak-Pavlovic, Thomas Rudel, Martin Fraunholz |
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URN: | urn:nbn:de:bvb:20-opus-232292 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | Frontiers in Cellular and Infection Microbiology |
ISSN: | 2235-2988 |
Year of Completion: | 2021 |
Volume: | 11 |
Article Number: | 644750 |
Source: | Frontiers in Cellular and Infection Microbiology 2021, 11:644750. DOI: 10.3389/fcimb.2021.644750 |
DOI: | https://doi.org/10.3389/fcimb.2021.644750 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Tag: | autophagosomes; endosomes; expansion microscopy; high-resolution imaging; host-pathogen interaction |
Release Date: | 2022/02/03 |
Open-Access-Publikationsfonds / Förderzeitraum 2021 | |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |