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CD28 costimulation of T helper 1 cells enhances cytokine release in vivo

Please always quote using this URN: urn:nbn:de:bvb:20-opus-176726
  • Compared to naive T cells, differentiated T cells are thought to be less dependent on CD28 costimulation for full activation. To revisit the role of CD28 costimulation in mouse T cell recall responses, we adoptively transferred in vitro generated OT-II T helper (Th) 1 cells into C57BL/6 mice (Thy1.2\(^{+}\)) and then either blocked CD28–ligand interactions with Fab fragments of the anti-CD28 monoclonal antibody (mAb) E18 or deleted CD28 expression using inducible CD28 knock-out OT-II mice as T cell donors. After injection of ovalbumin proteinCompared to naive T cells, differentiated T cells are thought to be less dependent on CD28 costimulation for full activation. To revisit the role of CD28 costimulation in mouse T cell recall responses, we adoptively transferred in vitro generated OT-II T helper (Th) 1 cells into C57BL/6 mice (Thy1.2\(^{+}\)) and then either blocked CD28–ligand interactions with Fab fragments of the anti-CD28 monoclonal antibody (mAb) E18 or deleted CD28 expression using inducible CD28 knock-out OT-II mice as T cell donors. After injection of ovalbumin protein in adjuvant into the recipient mice we observed that systemic interferon (IFN)γ release strongly depended on CD28 costimulation of the Th1 cells, while secondary clonal expansion was not reduced in the absence of CD28 costimulation. For human memory CD4\(^{+}\) T cell responses we also noted that cytokine release was reduced upon inhibition of CD28 costimulation. Together, our data highlight the so far underestimated role of CD28 costimulation for the reactivation of fully differentiated CD4\(^{+}\) T cells.show moreshow less

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Metadaten
Author: Daniela Langenhorst, Stephanie Haack, Selina Göb, Anna Uri, Fred Lühder, Bernhard Vanhove, Thomas Hünig, Niklas Beyersdorf
URN:urn:nbn:de:bvb:20-opus-176726
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Virologie und Immunbiologie
Language:English
Parent Title (English):Frontiers in Immunology
Year of Completion:2018
Volume:9
Issue:1060
Source:Frontiers in Immunology 2018, Volume 9, Article 1060. DOI: 10.3389/fimmu.2018.01060
DOI:https://doi.org/10.3389/fimmu.2018.01060
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:CD28 costimulation; CD4\(^{+}\) T helper cells; T helper 1 cells; antigenic recall; cytokine secretion; human; mouse
Release Date:2019/02/26
Collections:Open-Access-Publikationsfonds / Förderzeitraum 2018
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International