The salt-and-pepper pattern in mouse blastocysts is compatible with signaling beyond the nearest neighbors
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- Summary Embryos develop in a concerted sequence of spatiotemporal arrangements of cells. In the preimplantation mouse embryo, the distribution of the cells in the inner cell mass evolves from a salt-and-pepper pattern to spatial segregation of two distinct cell types. The exact properties of the salt-and-pepper pattern have not been analyzed so far. We investigate the spatiotemporal distribution of NANOG- and GATA6-expressing cells in the ICM of the mouse blastocysts with quantitative three-dimensional single-cell-based neighborhood analyses.Summary Embryos develop in a concerted sequence of spatiotemporal arrangements of cells. In the preimplantation mouse embryo, the distribution of the cells in the inner cell mass evolves from a salt-and-pepper pattern to spatial segregation of two distinct cell types. The exact properties of the salt-and-pepper pattern have not been analyzed so far. We investigate the spatiotemporal distribution of NANOG- and GATA6-expressing cells in the ICM of the mouse blastocysts with quantitative three-dimensional single-cell-based neighborhood analyses. A combination of spatial statistics and agent-based modeling reveals that the cell fate distribution follows a local clustering pattern. Using ordinary differential equations modeling, we show that this pattern can be established by a distance-based signaling mechanism enabling cells to integrate information from the whole inner cell mass into their cell fate decision. Our work highlights the importance of longer-range signaling to ensure coordinated decisions in groups of cells to successfully build embryos. Highlights • The local cell neighborhood and global ICM population composition correlate • ICM cells show characteristics of local clustering in early and mid mouse blastocysts • ICM patterning requires integration of signals from cells beyond the first neighbors…
Autor(en): | Sabine C. Fischer, Simon Schardt, Joaquín Lilao-Garzón, Silvia Muñoz-Descalzo |
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URN: | urn:nbn:de:bvb:20-opus-350184 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Center for Computational and Theoretical Biology |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | iScience |
Erscheinungsjahr: | 2023 |
Band / Jahrgang: | 26 |
Heft / Ausgabe: | 11 |
Aufsatznummer: | 108106 |
Originalveröffentlichung / Quelle: | iScience (2023) 26:11, 108106. DOI: 10.1016/j.isci.2023.108106 |
DOI: | https://doi.org/10.1016/j.isci.2023.108106 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Freie Schlagwort(e): | ICM cells; cellular physiology; developmental biology; local cell neighborhood; mouse blastocysts; salt-and-pepper pattern; signaling |
Datum der Freischaltung: | 16.04.2024 |
EU-Projektnummer / Contract (GA) number: | 2020010013 |
OpenAIRE: | OpenAIRE |
Lizenz (Deutsch): | CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International |