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The prototype foamy virus protease is active independently of the integrase domain
Please always quote using this URN: urn:nbn:de:bvb:20-opus-75370
- Background: Recently, contradictory results on foamy virus protease activity were published. While our own results indicated that protease activity is regulated by the viral RNA, others suggested that the integrase is involved in the regulation of the protease. Results: To solve this discrepancy we performed additional experiments showing that the protease-reverse transcriptase (PR-RT) exhibits protease activity in vitro and in vivo, which is independent of the integrase domain. In contrast, Pol incorporation, and therefore PR activity in theBackground: Recently, contradictory results on foamy virus protease activity were published. While our own results indicated that protease activity is regulated by the viral RNA, others suggested that the integrase is involved in the regulation of the protease. Results: To solve this discrepancy we performed additional experiments showing that the protease-reverse transcriptase (PR-RT) exhibits protease activity in vitro and in vivo, which is independent of the integrase domain. In contrast, Pol incorporation, and therefore PR activity in the viral context, is dependent on the integrase domain. To further analyse the regulation of the protease, we incorporated Pol in viruses by expressing a GagPol fusion protein, which supported near wild-type like infectivity. A GagPR-RT fusion, lacking the integrase domain, also resulted in wild-type like Gag processing, indicating that the integrase is dispensable for viral Gag maturation. Furthermore, we demonstrate with a trans-complementation assays that the PR in the context of the PR-RT protein supports in trans both, viral maturation and infectivity. Conclusion: We provide evidence that the FV integrase is required for Pol encapsidation and that the FV PR activity is integrase independent. We show that an active PR can be encapsidated in trans as a GagPR-RT fusion protein.…
Author: | Ralf Spannaus, Maximilian J. Hartl, Birgitta M. Wöhrl, Axel Rethwilm, Jochen Bodem |
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URN: | urn:nbn:de:bvb:20-opus-75370 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Virologie und Immunbiologie |
Language: | English |
Year of Completion: | 2012 |
Source: | In: Retrovirology (2012) 9: 41, doi:10.1186/1742-4690-9-41 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
GND Keyword: | Medizin |
Tag: | Foamy virus; GagPol fusion protein; Integrase; PARM; Regulation of protease activity |
Release Date: | 2013/03/26 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2012 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |