Chromosome Banding in Amphibia. XXXIII. Demonstration of 5-Methylcytosine-Rich Heterochromatin in Anura
Please always quote using this URN: urn:nbn:de:bvb:20-opus-199022
- An experimental approach using monoclonal anti-5-methylcytosine (5-MeC) antibodies and indirect immunofluorescence was elaborated for detecting 5-MeC-rich chromosome regions in anuran chromosomes. This technique was applied to mitotic metaphases of 6 neotropical frog species belonging to 6 genera and 4 families. The hypermethylation patterns were compared with a variety of banding patterns obtained by conventional banding techniques. The hypermethylated DNA sequences are species-specific and located exclusively in constitutive heterochromatin.An experimental approach using monoclonal anti-5-methylcytosine (5-MeC) antibodies and indirect immunofluorescence was elaborated for detecting 5-MeC-rich chromosome regions in anuran chromosomes. This technique was applied to mitotic metaphases of 6 neotropical frog species belonging to 6 genera and 4 families. The hypermethylation patterns were compared with a variety of banding patterns obtained by conventional banding techniques. The hypermethylated DNA sequences are species-specific and located exclusively in constitutive heterochromatin. They are found in centromeric, pericentromeric, telomeric, and interstitial positions of the chromosomes and adjacent to nucleolus organizer regions. 5-MeC-rich DNA sequences can be embedded both in AT- and GC-rich repetitive DNA. The experimental parameters that have major influence on the reproducibility and quality of the anti-5-MeC antibody labeling are discussed.…
Author: | Michael Schmid, Claus Steinlein |
---|---|
URN: | urn:nbn:de:bvb:20-opus-199022 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Humangenetik |
Language: | English |
Parent Title (English): | Cytogenetic and Genome Research |
ISSN: | 1424-8581 |
ISSN: | 1424-859X |
Year of Completion: | 2016 |
Volume: | 148 |
Issue: | 1 |
Pagenumber: | 35-43 |
Source: | Cytogenetic and Genome Research 2016;148(1):35–43. DOI: 10.1159/000446141 |
DOI: | https://doi.org/10.1159/000446141 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/27160320 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | 5-Methylcytosine; Anura; heterochromatin; hypermethylated DNA; immunofluorescence |
Release Date: | 2021/08/20 |
Date of first Publication: | 2016/04/30 |
Note: | This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively. |
Licence (German): | Deutsches Urheberrecht |