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Investigating bioconjugation by atomic force microscopy

Please always quote using this URN: urn:nbn:de:bvb:20-opus-129477
  • Nanotechnological applications increasingly exploit the selectivity and processivity of biological molecules. Integration of biomolecules such as proteins or DNA into nano-systems typically requires their conjugation to surfaces, for example of carbon-nanotubes or fluorescent quantum dots. The bioconjugated nanostructures exploit the unique strengths of both their biological and nanoparticle components and are used in diverse, future oriented research areas ranging from nanoelectronics to biosensing and nanomedicine. Atomic force microscopyNanotechnological applications increasingly exploit the selectivity and processivity of biological molecules. Integration of biomolecules such as proteins or DNA into nano-systems typically requires their conjugation to surfaces, for example of carbon-nanotubes or fluorescent quantum dots. The bioconjugated nanostructures exploit the unique strengths of both their biological and nanoparticle components and are used in diverse, future oriented research areas ranging from nanoelectronics to biosensing and nanomedicine. Atomic force microscopy imaging provides valuable, direct insight for the evaluation of different conjugation approaches at the level of the individual molecules. Recent technical advances have enabled high speed imaging by AFM supporting time resolutions sufficient to follow conformational changes of intricately assembled nanostructures in solution. In addition, integration of AFM with different spectroscopic and imaging approaches provides an enhanced level of information on the investigated sample. Furthermore, the AFM itself can serve as an active tool for the assembly of nanostructures based on bioconjugation. AFM is hence a major workhorse in nanotechnology; it is a powerful tool for the structural investigation of bioconjugation and bioconjugation-induced effects as well as the simultaneous active assembly and analysis of bioconjugation-based nanostructures.show moreshow less

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Metadaten
Author: Ingrid Tessmer, Parminder Kaur, Jiangguo Lin, Hong Wang
URN:urn:nbn:de:bvb:20-opus-129477
Document Type:Journal article
Faculties:Fakultät für Biologie / Rudolf-Virchow-Zentrum
Language:English
Parent Title (English):Journal of Nanobiotechnology
Year of Completion:2013
Volume:11
Issue:25
Source:Journal of Nanobiotechnology 2013, 11:25. doi:10.1186/1477-3155-11-25
DOI:https://doi.org/10.1186/1477-3155-11-25
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:DNA origami; atomic force microscopy (AFM); bioconjugation; biosensors; nanoelectronics; nanolithography; nanomedicine; nanorobot; nanotechnology; single molecule
Release Date:2016/06/27
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung