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The proteome profiles of the olfactory bulb of juvenile, adult and aged rats - an ontogenetic study

Please always quote using this URN: urn:nbn:de:bvb:20-opus-144073
  • Background: In this study, we searched for proteins that change their expression in the olfactory bulb (oB) of rats during ontogenesis. Up to now, protein expression differences in the developing animal are not fully understood. Our investigation focused on the question whether specific proteins exist which are only expressed during different development stages. This might lead to a better characterization of the microenvironment and to a better determination of factors and candidates that influence the differentiation of neuronal progenitorBackground: In this study, we searched for proteins that change their expression in the olfactory bulb (oB) of rats during ontogenesis. Up to now, protein expression differences in the developing animal are not fully understood. Our investigation focused on the question whether specific proteins exist which are only expressed during different development stages. This might lead to a better characterization of the microenvironment and to a better determination of factors and candidates that influence the differentiation of neuronal progenitor cells. Results: After analyzing the samples by two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), it could be shown that the number of expressed proteins differs depending on the developmental stages. Especially members of the functional classes, like proteins of biosynthesis, regulatory proteins and structural proteins, show the highest differential expression in the stages of development analyzed. Conclusion: In this study, quantitative changes in the expression of proteins in the oB at different developmental stages (postnatal days (P) 7, 90 and 637) could be observed. Furthermore, the expression of many proteins was found at specific developmental stages. It was possible to identify these proteins which are involved in processes like support of cell migration and differentiation.show moreshow less

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Metadaten
Author: Michael Wille, Antje Schümann, Michael Kreutzer, Michael O Glocker, Andreas Wree, Grit Mutzbauer, Oliver Schmitt
URN:urn:nbn:de:bvb:20-opus-144073
Document Type:Journal article
Faculties:Medizinische Fakultät / Pathologisches Institut
Language:English
Parent Title (English):Proteome Science
Year of Completion:2015
Volume:13
Issue:8
Source:Proteome Science (2015) 13:8. DOI: 10.1186/s12953-014-0058-x
DOI:https://doi.org/10.1186/s12953-014-0058-x
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:axonally transported proteins; brain; cerebral cortex; development; goldfish optic nerve; growth-associated protein; hippocampal stem cells; messenger-RNA transport; neuronal differentiation; olfactory bulb; postnatal development; proteomics; rat; regional development; subventricular zone
Release Date:2018/06/04
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International