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Identifizierung von Biomarkern der Belastung von Mundschleimhautzellen mit Schwermetallen und polyzyklischen aromatischen Kohlenwasserstoffen
Identification of biomarkers for exposure of human oral mucosa to heavy metals or polycyclic aromatic hydrocarbons
Please always quote using this URN: urn:nbn:de:bvb:20-opus-73302
- Menschliche Mundschleimheut wurde ex-vivo gegenüber Schwermetallen (Blei) oder polyzyklischen aromatischen Kohlenwasserstoffen (Benzopyren) für Zeiten von 5Min. bis 360Min exponiert. Immunhistochemisch wurdne im Anschluss Marker für Apoptose, oxitaven und nitrogenen Stress untersucht. Hierbei zeigten sich jeweils charakteristische Veränderungen für aktive Caspase-3, 3-Nitrotyrosine und 8-epi-PGF2alpha. Proben von Rauchern wurden mit Nichtraucherproben verglichen und zeigten verminderte Werte für oxidativen und nitrogenen Stress.
- In this study samples of human oral mucosa were ex-vivo exposed to lead or benzopyrene to further investigate the influence of heavy metals and polycyclic aromatic hydrocarbons on apoptosis and generation of oxidative and nitrosative stress in human oral mucosa. Immunhistochemical staining was done for active-caspase-3, 8-epi-PGF2a and 3-nitrotyrosine as biomarkers for apoptosis as well as oxidative and nitrosative stress. Samples obtained from smokers where furthermore compared to non-smokers' samples. Activation of apoptosis and generation ofIn this study samples of human oral mucosa were ex-vivo exposed to lead or benzopyrene to further investigate the influence of heavy metals and polycyclic aromatic hydrocarbons on apoptosis and generation of oxidative and nitrosative stress in human oral mucosa. Immunhistochemical staining was done for active-caspase-3, 8-epi-PGF2a and 3-nitrotyrosine as biomarkers for apoptosis as well as oxidative and nitrosative stress. Samples obtained from smokers where furthermore compared to non-smokers' samples. Activation of apoptosis and generation of oxidative and nitrosative stress already occured in oral mucosa cells after short-term, one-time exposure to lead or benzopyrene and is not limited to chronic exposure. In smokers' samples there seems to be a reduced generation of reactive oxygen or nitrogen species after exposure to benzopyrene.…
Author: | Andreas Wannhoff |
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URN: | urn:nbn:de:bvb:20-opus-73302 |
Document Type: | Doctoral Thesis |
Granting Institution: | Universität Würzburg, Medizinische Fakultät |
Faculties: | Medizinische Fakultät / Klinik und Poliklinik für Mund-, Kiefer- und Plastische Gesichtschirurgie |
Date of final exam: | 2012/08/02 |
Language: | German |
Year of Completion: | 2011 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
GND Keyword: | Mundschleimhaut; Benzopyren; Polycyclische Aromaten; Blei; Schwermetall; Apoptosis; Oxidativer Stress |
Tag: | apoptosis; benzopyrene; human oral mucosa; lead; oxidative stress |
Release Date: | 2013/11/13 |
Advisor: | Priv.-Doz. Dr. med. Dr. med. dent. Tobias Reuther |
Licence (German): | Deutsches Urheberrecht |