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Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68
Please always quote using this URN: urn:nbn:de:bvb:20-opus-141503
- Background: Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo. Methods: In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also testedBackground: Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo. Methods: In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain. In order to identify correlates of permissity to viral infection, we measured transcriptional profiles of the cell lines prior infection. Results: We observed highly heterogeneous permissivity to VACV infection amongst the cell lines. The heterogeneity of permissivity was independent of tissue with the exception of B cell derivation. Cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain and a significant correlation was found suggesting a common permissive phenotype. While no clear transcriptional pattern could be identified as predictor of permissivity to infection, some associations were observed suggesting multifactorial basis permissivity to viral infection. Conclusions: Our findings have implications for the design of oncolytic therapies for cancer and offer insights into the nature of permissivity of tumor cells to viral infection.…
Author: | Maria Libera Ascierto, Andrea Worschech, Zhiya Yu, Sharon Adams, Jennifer Reinboth, Nanhai G Chen, Zoltan Pos, Rahul Roychoudhuri, Giovanni Di Pasquale, Davide Bedognetti, Lorenzo Uccellini, Fabio Rossano, Paolo A Ascierto, David F Stroncek, Nicholas P Restifo, Ena Wang, Aladar A Szalay, Francesco M Marincola |
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URN: | urn:nbn:de:bvb:20-opus-141503 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Medizinische Klinik und Poliklinik I |
Fakultät für Chemie und Pharmazie / Lehrstuhl für Biochemie | |
Language: | English |
Parent Title (English): | BMC Cancer |
Year of Completion: | 2011 |
Volume: | 11 |
Issue: | 451 |
Pagenumber: | 1-14 |
Source: | BMC Cancer 2011, 11:451 |
DOI: | https://doi.org/10.1186/1471-2407-11-451 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | CD9; adenovirus; gene-therapy; identification; infection; panel; receptor |
Release Date: | 2019/01/09 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |