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Polyoxazolines with a vicinally double-bioactivated terminus for biomacromolecular affinity assessment
Please always quote using this URN: urn:nbn:de:bvb:20-opus-239530
- Interactions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugatesInteractions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugates with a defined double bioactivation at the chain end. The target architecture is the vicinal attachment of two biomolecule residues to the α telechelic end point of a polymer and a thioether group at the ω chain end for fixating the conjugate to a gold sensor chip surface. As proof-of-principle studies for affinity measurements, we demonstrate the interaction between covalently bound mannose and ConA in surface acoustic wave (SAW) and surface plasmon resonance (SPR) experiments.…
Author: | Florian Pinzner, Thorsten Keller, Jürgen Mut, Julian Bechold, Jürgen Seibel, Jürgen Groll |
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URN: | urn:nbn:de:bvb:20-opus-239530 |
Document Type: | Journal article |
Faculties: | Fakultät für Chemie und Pharmazie / Institut für Organische Chemie |
Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde | |
Language: | English |
Parent Title (English): | Sensors |
ISSN: | 1424-8220 |
Year of Completion: | 2021 |
Volume: | 21 |
Issue: | 9 |
Article Number: | 3153 |
Source: | Sensors (2021) 21:9, 3153. https://doi.org/10.3390/s21093153 |
DOI: | https://doi.org/10.3390/s21093153 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Tag: | functionalization; lectin; polyoxazolines |
Release Date: | 2022/07/13 |
Date of first Publication: | 2021/05/01 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |