Foxp3\(^+\) Regulatory T Cells Control Persistence of Viral CNS Infection
Please always quote using this URN: urn:nbn:de:bvb:20-opus-134248
- We earlier established a model of a persistent viral CNS infection using two week old immunologically normal (genetically unmodified) mice and recombinant measles virus (MV). Using this model infection we investigated the role of regulatory T cells (Tregs) as regulators of the immune response in the brain, and assessed whether the persistent CNS infection can be modulated by manipulation of Tregs in the periphery. CD4\(^+\) CD25\(^+\) Foxp3\(^+\) Tregs were expanded or depleted during the persistent phase of the CNS infection, and theWe earlier established a model of a persistent viral CNS infection using two week old immunologically normal (genetically unmodified) mice and recombinant measles virus (MV). Using this model infection we investigated the role of regulatory T cells (Tregs) as regulators of the immune response in the brain, and assessed whether the persistent CNS infection can be modulated by manipulation of Tregs in the periphery. CD4\(^+\) CD25\(^+\) Foxp3\(^+\) Tregs were expanded or depleted during the persistent phase of the CNS infection, and the consequences for the virus-specific immune response and the extent of persistent infection were analyzed. Virus-specific CD8\(^+\) T cells predominantly recognising the H-2D(b)-presented viral hemagglutinin epitope MV-H22-30 (RIVINREHL) were quantified in the brain by pentamer staining. Expansion of Tregs after intraperitoneal (i.p.) application of the superagonistic anti-CD28 antibody D665 inducing transient immunosuppression caused increased virus replication and spread in the CNS. In contrast, depletion of Tregs using diphtheria toxin (DT) in DEREG (depletion of regulatory T cells)-mice induced an increase of virus-specific CD8\(^+\) effector T cells in the brain and caused a reduction of the persistent infection. These data indicate that manipulation of Tregs in the periphery can be utilized to regulate virus persistence in the CNS.…
Author: | Dajana Reuter, Tim Sparwasser, Thomas Hünig, Jürgen Schneider-Schaulies |
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URN: | urn:nbn:de:bvb:20-opus-134248 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Virologie und Immunbiologie |
Language: | English |
Parent Title (English): | PLoS One |
Year of Completion: | 2012 |
Volume: | 7 |
Issue: | 3 |
Pagenumber: | e33989 |
Source: | PLoS ONE 7(3): e33989. doi:10.1371/journal.pone.0033989 |
DOI: | https://doi.org/10.1371/journal.pone.0033989 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | CD4(+); antigen presentation; brain; central-nervous-system; gamma-interferon; measles virus; mice; retroviral infection; subacute sclerosing-panencephalitis; virus-induced encephalitis |
Release Date: | 2017/06/13 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |