Nucleotide sequence analysis of a cloned DNA fragment from human cells reveals homology to retrotransposons
Please always quote using this URN: urn:nbn:de:bvb:20-opus-61525
- During molecular cloning of proviral DNA of human. spumaretroVirus, various recombinant clones were estabUshed and analyzed. Blot hybridization revealed that one of the recoinbinant plasmids bad the characteristic features of a member of the long interspersed repetitive sequences famlly. The DNA element was analyzed by restrictioil mapping and nuelootide sequencing. It showed a high degree of amino acid sequence homology of 54.3% when conipared with the 5'-terminal part of the pol gelie product of the murine retrotransposon LIMd. The 3' regionDuring molecular cloning of proviral DNA of human. spumaretroVirus, various recombinant clones were estabUshed and analyzed. Blot hybridization revealed that one of the recoinbinant plasmids bad the characteristic features of a member of the long interspersed repetitive sequences famlly. The DNA element was analyzed by restrictioil mapping and nuelootide sequencing. It showed a high degree of amino acid sequence homology of 54.3% when conipared with the 5'-terminal part of the pol gelie product of the murine retrotransposon LIMd. The 3' region of the cloned DNA element encodes proteins witb an even higher degree of homology of 67.4% in comparison to the corresponding parts of a member of the primate Kpnl sequence family.…
Author: | Rolf M. Flügel, Bernd Maurer, Helmut Bannert, Axel Rethwilm, Paul Schnitzler, Gholamreza Darai |
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URN: | urn:nbn:de:bvb:20-opus-61525 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Virologie und Immunbiologie |
Language: | English |
Year of Completion: | 1987 |
Source: | In: MOLECULAR AND CELLULAR BIOLOGY (1987) 7, 1, 231-236 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
GND Keyword: | Virologie |
Release Date: | 2011/09/17 |
Licence (German): | Deutsches Urheberrecht |