Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice
Please always quote using this URN: urn:nbn:de:bvb:20-opus-179745
- Atherosclerotic lesions that critically narrow the artery can necessitate an angioplasty and stent implantation. Long-term therapeutic effects, however, are limited by excessive arterial remodeling. We here employed a miniaturized nitinol-stent coated with star-shaped polyethylenglycole (star-PEG), and evaluated its bio-functionalization with RGD and CXCL1 for improving in-stent stenosis after implantation into carotid arteries of mice. Nitinol foils or stents (bare metal) were coated with star-PEG, and bio-functionalized with RGD, orAtherosclerotic lesions that critically narrow the artery can necessitate an angioplasty and stent implantation. Long-term therapeutic effects, however, are limited by excessive arterial remodeling. We here employed a miniaturized nitinol-stent coated with star-shaped polyethylenglycole (star-PEG), and evaluated its bio-functionalization with RGD and CXCL1 for improving in-stent stenosis after implantation into carotid arteries of mice. Nitinol foils or stents (bare metal) were coated with star-PEG, and bio-functionalized with RGD, or RGD/CXCL1. Cell adhesion to star-PEG-coated nitinol foils was unaltered or reduced, whereas bio-functionalization with RGD but foremost RGD/CXCL1 increased adhesion of early angiogenic outgrowth cells (EOCs) and endothelial cells but not smooth muscle cells when compared with bare metal foils. Stimulation of cells with RGD/CXCL1 furthermore increased the proliferation of EOCs. In vivo, bio-functionalization with RGD/CXCL1 significantly reduced neointima formation and thrombus formation, and increased re-endothelialization in apoE\(^{-/-}\) carotid arteries compared with bare-metal nitinol stents, star-PEG-coated stents, and stents bio-functionalized with RGD only. Bio-functionalization of star-PEG-coated nitinol-stents with RGD/CXCL1 reduced in-stent neointima formation. By supporting the adhesion and proliferation of endothelial progenitor cells, RGD/CXCL1 coating of stents may help to accelerate endothelial repair after stent implantation, and thus may harbor the potential to limit the complication of in-stent restenosis in clinical approaches.…
Author: | Sakine Simsekyilmaz, Elisa A. Liehn, Stefan Weinandy, Fabian Schreiber, Remco T. A. Megens, Wendy Theelen, Ralf Smeets, Stefan Jockenhövel, Thomas Gries, Martin Möller, Doris Klee, Christian Weber, Alma Zernecke |
---|---|
URN: | urn:nbn:de:bvb:20-opus-179745 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Experimentelle Biomedizin |
Language: | English |
Parent Title (English): | PLoS ONE |
Year of Completion: | 2016 |
Volume: | 11 |
Issue: | 5 |
Article Number: | e0155829 |
Source: | PLoS ONE 2016, 11(5):e0155829. DOI:10.1371/journal.pone.0155829 |
DOI: | https://doi.org/10.1371/journal.pone.0155829 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | carotid arteries; coatings; endothelial cells; fluorescence microscopy; mice; polymers; stem cells; stent implantation |
Release Date: | 2020/12/11 |
EU-Project number / Contract (GA) number: | 249929 |
OpenAIRE: | OpenAIRE |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |