Fluorescence localization microscopy
Please always quote using this URN: urn:nbn:de:bvb:20-opus-124416
- Localization microscopy techniques are super-resolution fluorescence imaging methods based on the detection of individual molecules. Despite the relative simplicity of the microscope setups and the availability of commercial instruments, localization microscopy faces unique challenges. While achieving super-resolution is now routine, issues concerning data analysis and interpretation mean that revealing novel biological insights is not. Here, we outline why data analysis and the design of robust test samples may hold the key to harness the fullLocalization microscopy techniques are super-resolution fluorescence imaging methods based on the detection of individual molecules. Despite the relative simplicity of the microscope setups and the availability of commercial instruments, localization microscopy faces unique challenges. While achieving super-resolution is now routine, issues concerning data analysis and interpretation mean that revealing novel biological insights is not. Here, we outline why data analysis and the design of robust test samples may hold the key to harness the full potential of localization microscopy.…
Author: | Dylan M. Owen, Markus Sauer, Katharina Gaus |
---|---|
URN: | urn:nbn:de:bvb:20-opus-124416 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | Communicative & Integrative Biology |
Year of Completion: | 2012 |
Volume: | 5 |
Issue: | 4 |
Pagenumber: | 345-349 |
Source: | Communicative & Integrative Biology 5:4, 345–349. doi:10.4161/cib.20348 |
DOI: | https://doi.org/10.4161/cib.20348 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Release Date: | 2016/01/19 |
Licence (German): | CC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell |