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The effect of skin fatty acids on Staphylococcus aureus
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-121657
- Staphylococcus aureus is a commensal of the human nose and skin. Human skin fatty acids, in particular cis-6-hexadecenoic acid (C-6-H), have high antistaphylococcal activity and can inhibit virulence determinant production. Here, we show that sub-MIC levels of C-6-H result in induction of increased resistance. The mechanism(s) of C-6-H activity was investigated by combined transcriptome and proteome analyses. Proteome analysis demonstrated a pleiotropic effect of C-6-H on virulence determinant production. In response to C-6-H, transcriptomicsStaphylococcus aureus is a commensal of the human nose and skin. Human skin fatty acids, in particular cis-6-hexadecenoic acid (C-6-H), have high antistaphylococcal activity and can inhibit virulence determinant production. Here, we show that sub-MIC levels of C-6-H result in induction of increased resistance. The mechanism(s) of C-6-H activity was investigated by combined transcriptome and proteome analyses. Proteome analysis demonstrated a pleiotropic effect of C-6-H on virulence determinant production. In response to C-6-H, transcriptomics revealed altered expression of over 500 genes, involved in many aspects of virulence and cellular physiology. The expression of toxins (hla, hlb, hlgBC) was reduced, whereas that of host defence evasion components (cap, sspAB, katA) was increased. In particular, members of the SaeRS regulon had highly reduced expression, and the use of specific mutants revealed that the effect on toxin production is likely mediated via SaeRS…
Autor(en): | Yvonne Neumann, Knut Ohlsen, Stefanie Donat, Susanne Engelmann, Harald Kusch, Dirk Albrecht, Michael Cartron, Alexander Hurd, Simon J. Foster |
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URN: | urn:nbn:de:bvb:20-opus-121657 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Molekulare Infektionsbiologie |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Archives of Microbiology |
Erscheinungsjahr: | 2015 |
Band / Jahrgang: | 197 |
Seitenangabe: | 245-67 |
Originalveröffentlichung / Quelle: | Archives of Microbiology (2015) 197:245–267 DOI 10.1007/s00203-014-1048-1 |
DOI: | https://doi.org/10.1007/s00203-014-1048-1 |
PubMed-ID: | https://pubmed.ncbi.nlm.nih.gov/25325933 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | C-6-H; S. aureus; resistance; skin fatty acid |
Datum der Freischaltung: | 19.02.2016 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung |