Unraveling the hidden temporal range of fast β2-adrenergic receptor mobility by time-resolved fluorescence
Please always quote using this URN: urn:nbn:de:bvb:20-opus-301140
- G-protein-coupled receptors (GPCRs) are hypothesized to possess molecular mobility over a wide temporal range. Until now the temporal range has not been fully accessible due to the crucially limited temporal range of available methods. This in turn, may lead relevant dynamic constants to remain masked. Here, we expand this dynamic range by combining fluorescent techniques using a spot confocal setup. We decipher mobility constants of β\(_{2}\)-adrenergic receptor over a wide time range (nanosecond to second). Particularly, a translationalG-protein-coupled receptors (GPCRs) are hypothesized to possess molecular mobility over a wide temporal range. Until now the temporal range has not been fully accessible due to the crucially limited temporal range of available methods. This in turn, may lead relevant dynamic constants to remain masked. Here, we expand this dynamic range by combining fluorescent techniques using a spot confocal setup. We decipher mobility constants of β\(_{2}\)-adrenergic receptor over a wide time range (nanosecond to second). Particularly, a translational mobility (10 µm\(^{2}\)/s), one order of magnitude faster than membrane associated lateral mobility that explains membrane protein turnover and suggests a wider picture of the GPCR availability on the plasma membrane. And a so far elusive rotational mobility (1-200 µs) which depicts a previously overlooked dynamic component that, despite all complexity, behaves largely as predicted by the Saffman-Delbrück model.…
Author: | Ashwin Balakrishnan, Katherina Hemmen, Susobhan Choudhury, Jan-Hagen Krohn, Kerstin Jansen, Mike Friedrich, Gerti Beliu, Markus Sauer, Martin J. Lohse, Katrin G. Heinze |
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URN: | urn:nbn:de:bvb:20-opus-301140 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Fakultät für Biologie / Rudolf-Virchow-Zentrum | |
Language: | English |
Parent Title (English): | Communications Biology |
Year of Completion: | 2022 |
Volume: | 5 |
Issue: | 1 |
Article Number: | 176 |
Source: | Communications Biology 2022, 5(1):176. DOI: 10.1038/s42003-022-03106-4 |
DOI: | https://doi.org/10.1038/s42003-022-03106-4 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Tag: | G-protein-coupled receptors; molecular mobility; temporal range |
Release Date: | 2023/02/24 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2022 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |