Normal Platelet Integrin Function in Mice Lacking Hydrogen Peroxide-Induced Clone-5 (Hic-5)
Please always quote using this URN: urn:nbn:de:bvb:20-opus-125724
- Integrin αIIbβ3 plays a central role in the adhesion and aggregation of platelets and thus is essential for hemostasis and thrombosis. Integrin activation requires the transmission of a signal from the small cytoplasmic tails of the α or β subunit to the large extracellular domains resulting in conformational changes of the extracellular domains to enable ligand binding. Hydrogen peroxide-inducible clone-5 (Hic-5), a member of the paxillin family, serves as a focal adhesion adaptor protein associated with αIIbβ3 at its cytoplasmic tails.Integrin αIIbβ3 plays a central role in the adhesion and aggregation of platelets and thus is essential for hemostasis and thrombosis. Integrin activation requires the transmission of a signal from the small cytoplasmic tails of the α or β subunit to the large extracellular domains resulting in conformational changes of the extracellular domains to enable ligand binding. Hydrogen peroxide-inducible clone-5 (Hic-5), a member of the paxillin family, serves as a focal adhesion adaptor protein associated with αIIbβ3 at its cytoplasmic tails. Previous studies suggested Hic-5 as a novel regulator of integrin αIIbβ3 activation and platelet aggregation in mice. To assess this in more detail, we generated Hic-5-null mice and analyzed activation and aggregation of their platelets in vitro and in vivo. Surprisingly, lack of Hic-5 had no detectable effect on platelet integrin activation and function in vitro and in vivo under all tested conditions. These results indicate that Hic-5 is dispensable for integrin αIIbβ3 activation and consequently for arterial thrombosis and hemostasis in mice.…
Author: | Michael Popp, Ina Thielman, Bernhard Nieswandt, David Stegner |
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URN: | urn:nbn:de:bvb:20-opus-125724 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Rudolf-Virchow-Zentrum |
Language: | English |
Parent Title (English): | PLoS One |
Year of Completion: | 2015 |
Volume: | 10 |
Issue: | 7 |
Pagenumber: | e0133429 |
Source: | PLoS ONE 10(7): e0133429. doi:10.1371/journal. pone.0133429 |
DOI: | https://doi.org/10.1371/journal.pone.0133429 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | blood; fibrinogen; flow cytometry; integrins; platelet activation; platelet aggregation; platelets; thrombin |
Release Date: | 2016/02/02 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2015 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |