Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging
Please always quote using this URN: urn:nbn:de:bvb:20-opus-119730
- In recent years three-dimensional (3D) super-resolution fluorescence imaging by single-molecule localization (localization microscopy) has gained considerable interest because of its simple implementation and high optical resolution. Astigmatic and biplane imaging are experimentally simple methods to engineer a 3D-specific point spread function (PSF), but existing evaluation methods have proven problematic in practical application. Here we introduce the use of cubic B-splines to model the relationship of axial position and PSF width in theIn recent years three-dimensional (3D) super-resolution fluorescence imaging by single-molecule localization (localization microscopy) has gained considerable interest because of its simple implementation and high optical resolution. Astigmatic and biplane imaging are experimentally simple methods to engineer a 3D-specific point spread function (PSF), but existing evaluation methods have proven problematic in practical application. Here we introduce the use of cubic B-splines to model the relationship of axial position and PSF width in the above mentioned approaches and compare the performance with existing methods. We show that cubic B-splines are the first method that can combine precision, accuracy and simplicity.…
Author: | Sven Proppert, Steve Wolter, Thorge Holm, Theresa Klein, Sebastian van de Linde, Markus Sauer |
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URN: | urn:nbn:de:bvb:20-opus-119730 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | Optics Express |
ISSN: | 1094-4087 |
Year of Completion: | 2014 |
Volume: | 22 |
Issue: | 9 |
Pagenumber: | 10304-16 |
Source: | Optics Express 22,9 p.10304-16. DOI:10.1364/OE.22.010304 |
DOI: | https://doi.org/10.1364/OE.22.010304 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/24921733 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 50 Naturwissenschaften / 502 Verschiedenes |
Tag: | fluorescence microscopy; medical and biological imaging; superresolution three-dimensional microscopy |
Release Date: | 2015/11/12 |
Licence (German): | Deutsches Urheberrecht |