Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
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- At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-scanning electron microscopy (FIB-SEM), we volumetrically reconstructed the anterior sensory apparatus of C. elegans dauer larvae with unprecedented precision. We provide a detailed description of some neurons, focusing on structural details thatAt the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-scanning electron microscopy (FIB-SEM), we volumetrically reconstructed the anterior sensory apparatus of C. elegans dauer larvae with unprecedented precision. We provide a detailed description of some neurons, focusing on structural details that were unknown or unresolved by previously published studies. They include the following: (1) dauer-specific branches of the IL2 sensory neurons project into the periphery of anterior sensilla and motor or putative sensory neurons at the sub-lateral cords; (2) ciliated endings of URX sensory neurons are supported by both ILso and AMso socket cells near the amphid openings; (3) variability in amphid sensory dendrites among dauers; and (4) somatic RIP interneurons maintain their projection into the pharyngeal nervous system. Our results support the notion that dauer larvae structurally expand their sensory system to facilitate searching for more favorable environments.…
Autor(en): | Sebastian Britz, Sebastian Matthias Markert, Daniel Witvliet, Anna Maria Steyer, Sarah Tröger, Ben Mulcahy, Philip Kollmannsberger, Yannick Schwab, Mei Zhen, Christian Stigloher |
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URN: | urn:nbn:de:bvb:20-opus-249622 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Fakultät für Biologie / Center for Computational and Theoretical Biology | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Frontiers in Neuroanatomy |
ISSN: | 1662-5129 |
Erscheinungsjahr: | 2021 |
Band / Jahrgang: | 15 |
Aufsatznummer: | 732520 |
Originalveröffentlichung / Quelle: | Frontiers in Neuroanatomy (2021) 15:732520. doi: 10.3389/fnana.2021.732520 |
DOI: | https://doi.org/10.3389/fnana.2021.732520 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Freie Schlagwort(e): | 3D reconstruction; Caenorhabditis elegans (C. elegans); FIB-SEM; IL2 branching; amphids; dauer; neuroanatomy |
Datum der Freischaltung: | 11.02.2022 |
Datum der Erstveröffentlichung: | 05.11.2021 |
Open-Access-Publikationsfonds / Förderzeitraum 2021 | |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |