Production, purification and characterization of hemolysins from Listeria ivanovii and Listeria monocytogenes Sv4b.
Please always quote using this URN: urn:nbn:de:bvb:20-opus-60545
- In culture supematants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH-activated cytolysin, and which was therefore identified as Iisteriolysin 0 (LLO). In the case of L. ivanovii a second major supematant protein of molecular weight 24 kDa co-purified with LLO. However, the function of this protein has to be determined. In culture supematants of L. ivanovii a sphingomyelinase and a Iecithinase activity could beIn culture supematants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH-activated cytolysin, and which was therefore identified as Iisteriolysin 0 (LLO). In the case of L. ivanovii a second major supematant protein of molecular weight 24 kDa co-purified with LLO. However, the function of this protein has to be determined. In culture supematants of L. ivanovii a sphingomyelinase and a Iecithinase activity could be detected, both enzymatic activities together contributing to the pronounced hemolysis caused by L. ivanovii. The N-tenninal amino acid sequences of LLO and the 24 kDa from L. ivanovii are shown.…
Author: | Jürgen Kreft, Dorothee Funke, Albert Haas, Friedrich Lottspeich, Werner Goebel |
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URN: | urn:nbn:de:bvb:20-opus-60545 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Year of Completion: | 1989 |
Source: | In: FEMS Microbiology Letters (1989) 57, 2, S. 197 - 202. |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
GND Keyword: | Biologie |
Tag: | Hemolysin; Listeria |
Release Date: | 2011/09/02 |
Licence (German): | Deutsches Urheberrecht |