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Super-resolution microscopy of the synaptic active zone
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-148997
- Brain function relies on accurate information transfer at chemical synapses. At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Calcium channels are pivotal for the initiation of excitation-secretion coupling and, correspondingly, capture a central position at the AZ. Combining quantitative functional studies with modeling approaches has provided predictions of channel properties, numbers and evenBrain function relies on accurate information transfer at chemical synapses. At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Calcium channels are pivotal for the initiation of excitation-secretion coupling and, correspondingly, capture a central position at the AZ. Combining quantitative functional studies with modeling approaches has provided predictions of channel properties, numbers and even positions on the nanometer scale. However, elucidating the nanoscopic organization of the surrounding protein network requires direct ultrastructural access. Without this information, knowledge of molecular synaptic structure-function relationships remains incomplete. Recently, super-resolution microscopy (SRM) techniques have begun to enter the neurosciences. These approaches combine high spatial resolution with the molecular specificity of fluorescence microscopy. Here, we discuss how SRM can be used to obtain information on the organization of AZ proteins…
Autor(en): | Nadine Ehmann, Markus Sauer, Robert J. Kittel |
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URN: | urn:nbn:de:bvb:20-opus-148997 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Physiologisches Institut |
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Frontiers in Cellular Neuroscience |
Erscheinungsjahr: | 2015 |
Band / Jahrgang: | 9 |
Heft / Ausgabe: | 7 |
Originalveröffentlichung / Quelle: | Frontiers in Cellular Neuroscience 9:7 (2015). DOI: 10.3389/fncel.2015.00007 |
DOI: | https://doi.org/10.3389/fncel.2015.00007 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | Ca\(^{2+}\) channels; active zone; excitation-secretion coupling; neurotransmitter release; presynaptic calcium; structure-function relationships; super-resolution microscopy |
Datum der Freischaltung: | 23.11.2018 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |