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Influence of Staphylococcus aureus strain background on Sa3int phage life cycle switches
Please always quote using this URN: urn:nbn:de:bvb:20-opus-297209
- Staphylococcus aureus asymptomatically colonizes the nasal cavity of mammals, but it is also a leading cause of life-threatening infections. Most human nasal isolates carry Sa3 phages, which integrate into the bacterial hlb gene encoding a sphingomyelinase. The virulence factor-encoding genes carried by the Sa3-phages are highly human-specific, and most animal strains are Sa3 negative. Thus, both insertion and excision of the prophage could potentially confer a fitness advantage to S. aureus. Here, we analyzed the phage life cycle of two Sa3Staphylococcus aureus asymptomatically colonizes the nasal cavity of mammals, but it is also a leading cause of life-threatening infections. Most human nasal isolates carry Sa3 phages, which integrate into the bacterial hlb gene encoding a sphingomyelinase. The virulence factor-encoding genes carried by the Sa3-phages are highly human-specific, and most animal strains are Sa3 negative. Thus, both insertion and excision of the prophage could potentially confer a fitness advantage to S. aureus. Here, we analyzed the phage life cycle of two Sa3 phages, Φ13 and ΦN315, in different phage-cured S. aureus strains. Based on phage transfer experiments, strains could be classified into low (8325-4, SH1000, and USA300c) and high (MW2c and Newman-c) transfer strains. High-transfer strains promoted the replication of phages, whereas phage adsorption, integration, excision, or recA transcription was not significantly different between strains. RNASeq analyses of replication-deficient lysogens revealed no strain-specific differences in the CI/Mor regulatory switch. However, lytic genes were significantly upregulated in the high transfer strain MW2c Φ13 compared to strain 8325-4 Φ13. By transcriptional start site prediction, new promoter regions within the lytic modules were identified, which are likely targeted by specific host factors. Such host-phage interaction probably accounts for the strain-specific differences in phage replication and transfer frequency. Thus, the genetic makeup of the host strains may determine the rate of phage mobilization, a feature that might impact the speed at which certain strains can achieve host adaptation.…
Author: | Carina Rohmer, Ronja Dobritz, Dilek Tuncbilek-Dere, Esther Lehmann, David Gerlach, Shilpa Elizabeth George, Taeok Bae, Kay Nieselt, Christiane Wolz |
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URN: | urn:nbn:de:bvb:20-opus-297209 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | Viruses |
ISSN: | 1999-4915 |
Year of Completion: | 2022 |
Volume: | 14 |
Issue: | 11 |
Article Number: | 2471 |
Source: | Viruses (2022) 14:11, 2471. https://doi.org/10.3390/v14112471 |
DOI: | https://doi.org/10.3390/v14112471 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Tag: | Staphylococcus; gene regulation; hemolysin; induction; phage; virulence |
Release Date: | 2023/11/14 |
Date of first Publication: | 2022/11/08 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |