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Spt5 interacts genetically with Myc and is limiting for brain tumor growth in Drosophila
Please always quote using this URN: urn:nbn:de:bvb:20-opus-350197
- The transcription factor SPT5 physically interacts with MYC oncoproteins and is essential for efficient transcriptional activation of MYC targets in cultured cells. Here, we use Drosophila to address the relevance of this interaction in a living organism. Spt5 displays moderate synergy with Myc in fast proliferating young imaginal disc cells. During later development, Spt5-knockdown has no detectable consequences on its own, but strongly enhances eye defects caused by Myc overexpression. Similarly, Spt5-knockdown in larval type 2 neuroblastsThe transcription factor SPT5 physically interacts with MYC oncoproteins and is essential for efficient transcriptional activation of MYC targets in cultured cells. Here, we use Drosophila to address the relevance of this interaction in a living organism. Spt5 displays moderate synergy with Myc in fast proliferating young imaginal disc cells. During later development, Spt5-knockdown has no detectable consequences on its own, but strongly enhances eye defects caused by Myc overexpression. Similarly, Spt5-knockdown in larval type 2 neuroblasts has only mild effects on brain development and survival of control flies, but dramatically shrinks the volumes of experimentally induced neuroblast tumors and significantly extends the lifespan of tumor-bearing animals. This beneficial effect is still observed when Spt5 is knocked down systemically and after tumor initiation, highlighting SPT5 as a potential drug target in human oncology.…
Author: | Julia HofstetterORCiD, Ayoola OgunleyeORCiD, André Kutschke, Lisa Marie Buchholz, Elmar WolfORCiD, Thomas RaabeORCiD, Peter GallantORCiD |
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URN: | urn:nbn:de:bvb:20-opus-350197 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Klinische Biochemie und Pathobiochemie |
Language: | English |
Parent Title (English): | Life Science Alliance |
ISSN: | 2575-1077 |
Year of Completion: | 2024 |
Volume: | 7 |
Issue: | 1 |
Article Number: | e202302130 |
Source: | Life Science Alliance (2024) 7:1, e202302130. DOI: 10.26508/lsa.202302130 |
DOI: | https://doi.org/10.26508/lsa.202302130 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/37935464 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | Drosophila; Myc; brain tumor; transcription factor SPT5; tumor growth |
Release Date: | 2024/04/16 |
EU-Project number / Contract (GA) number: | 759139 |
OpenAIRE: | OpenAIRE |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |