Infarct growth precedes cerebral thrombosis following experimental stroke in mice
Please always quote using this URN: urn:nbn:de:bvb:20-opus-265791
- Ischemic stroke is among the leading causes of disability and death worldwide. In acute ischemic stroke, successful recanalization of occluded vessels is the primary therapeutic aim, but even if it is achieved, not all patients benefit. Although blockade of platelet aggregation did not prevent infarct progression, cerebral thrombosis as cause of secondary infarct growth has remained a matter of debate. As cerebral thrombi are frequently observed after experimental stroke, a thrombus-induced impairment of the brain microcirculation is consideredIschemic stroke is among the leading causes of disability and death worldwide. In acute ischemic stroke, successful recanalization of occluded vessels is the primary therapeutic aim, but even if it is achieved, not all patients benefit. Although blockade of platelet aggregation did not prevent infarct progression, cerebral thrombosis as cause of secondary infarct growth has remained a matter of debate. As cerebral thrombi are frequently observed after experimental stroke, a thrombus-induced impairment of the brain microcirculation is considered to contribute to tissue damage. Here, we combine the model of transient middle cerebral artery occlusion (tMCAO) with light sheet fluorescence microscopy and immunohistochemistry of brain slices to investigate the kinetics of thrombus formation and infarct progression. Our data reveal that tissue damage already peaks after 8 h of reperfusion following 60 min MCAO, while cerebral thrombi are only observed at later time points. Thus, cerebral thrombosis is not causative for secondary infarct growth during ischemic stroke.…
Author: | Vanessa Göb, Maximilian G. Voll, Lena Zimmermann, Katharina Hemmen, Guido Stoll, Bernhard Nieswandt, Michael K. Schuhmann, Katrin G. Heinze, David Stegner |
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URN: | urn:nbn:de:bvb:20-opus-265791 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Neurologische Klinik und Poliklinik |
Fakultät für Biologie / Rudolf-Virchow-Zentrum | |
Medizinische Fakultät / Institut für Experimentelle Biomedizin | |
Language: | English |
Parent Title (English): | Scientific Reports |
Year of Completion: | 2021 |
Volume: | 11 |
Issue: | 1 |
Article Number: | 22887 |
Source: | Scientific Reports (2021) 11:1, 22887. https://doi.org/10.1038/s41598-021-02360-6 |
DOI: | https://doi.org/10.1038/s41598-021-02360-6 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | cerebrovascular disorders; thrombosis |
Release Date: | 2022/05/03 |
Open-Access-Publikationsfonds / Förderzeitraum 2021 | |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |