Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence
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- The clinical and preclinical research of ischemic strokes (IS) is becoming increasingly comprehensive, especially with the emerging evidence of complex thrombotic and inflammatory interactions. Within these, the blood brain barrier (BBB) plays an important role in regulating the cellular interactions at the vascular interface and is therefore the object of many IS-related questions. Consequently, valid, economic and responsible methods to define BBB integrity are necessary. Therefore, we compared the three ex-vivo setups albumin Western blotThe clinical and preclinical research of ischemic strokes (IS) is becoming increasingly comprehensive, especially with the emerging evidence of complex thrombotic and inflammatory interactions. Within these, the blood brain barrier (BBB) plays an important role in regulating the cellular interactions at the vascular interface and is therefore the object of many IS-related questions. Consequently, valid, economic and responsible methods to define BBB integrity are necessary. Therefore, we compared the three ex-vivo setups albumin Western blot (WB), IgG WB and albumin intensity measurement (AIM) with regard to validity as well as temporal and economic efficacy. While the informative value of the three methods correlated significantly, the efficacy of the IgG WB dominated.…
Autor(en): | Maximilian FrankeORCiD, Michael BieberORCiD, Guido Stoll, Michael Klaus Schuhmann |
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URN: | urn:nbn:de:bvb:20-opus-234214 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Neurologische Klinik und Poliklinik |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Methods and Protocols |
ISSN: | 2409-9279 |
Erscheinungsjahr: | 2021 |
Band / Jahrgang: | 4 |
Heft / Ausgabe: | 1 |
Aufsatznummer: | 23 |
Originalveröffentlichung / Quelle: | Methods and Protocols 2021, 4(1), 23; https://doi.org/10.3390/mps4010023 |
DOI: | https://doi.org/10.3390/mps4010023 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | IgG; Western blot; albumin; blood brain barrier; immunohistochemistry; neuroinflammation; stroke; tMCAO |
Datum der Freischaltung: | 05.01.2022 |
Datum der Erstveröffentlichung: | 23.03.2021 |
Open-Access-Publikationsfonds / Förderzeitraum 2021 | |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |