Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp EG49 and Nocardiopsis sp RV163
Please always quote using this URN: urn:nbn:de:bvb:20-opus-116547
- Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by H-1 NMR. Ten known compounds, including angucycline, diketopiperazine and beta-carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of eitherTwo sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by H-1 NMR. Ten known compounds, including angucycline, diketopiperazine and beta-carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a, 6,11a, 12-tetrahydro-5a, 11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.…
Author: | Yousef Dashti, Tanja Grkovic, Usama Ramadan Abdelmohsen, Ute Hentschel, Ronald J. Quinn |
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URN: | urn:nbn:de:bvb:20-opus-116547 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | MARINE DRUGS |
ISSN: | 1660-3397 |
Year of Completion: | 2014 |
Volume: | 12 |
Issue: | 5 |
Pagenumber: | 3046-3059 |
Source: | Marine Drugs 2014, 12, 3046-3059; doi:10.3390/md12053046 |
DOI: | https://doi.org/10.3390/md12053046 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/24857962 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 593 Wirbellose des Meeres und der Meeresküste |
Tag: | A-D; NMR fingerprint; aspergillus fumigatus; bioactivity; biosynthesis; co-cultivation; induced metabolites; marine; natural products; sponge-associated actinomyetes |
Release Date: | 2015/07/30 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |