Covalently Cross-Linked Pig Gastric Mucin Hydrogels Prepared by Radical-Based Chain-Growth and Thiol-ene Mechanisms
Please always quote using this URN: urn:nbn:de:bvb:20-opus-318453
- Mucin, a high molecular mass hydrophilic glycoprotein, is the main component of mucus that coats every wet epithelium in animals. It is thus intrinsically biocompatible, and with its protein backbone and the o-glycosidic bound oligosaccharides, it contains a plethora of functional groups which can be used for further chemical modifications. Here, chain-growth and step-growth (thiol-ene) free-radical cross-linked hydrogels prepared from commercially available pig gastric mucin (PGM) are introduced and compared as cost-efficient and easilyMucin, a high molecular mass hydrophilic glycoprotein, is the main component of mucus that coats every wet epithelium in animals. It is thus intrinsically biocompatible, and with its protein backbone and the o-glycosidic bound oligosaccharides, it contains a plethora of functional groups which can be used for further chemical modifications. Here, chain-growth and step-growth (thiol-ene) free-radical cross-linked hydrogels prepared from commercially available pig gastric mucin (PGM) are introduced and compared as cost-efficient and easily accessible alternative to the more broadly applied bovine submaxillary gland mucin. For this, PGM is functionalized with photoreactive acrylate groups or allyl ether moieties, respectively. Whereas homopolymerization of acrylate-functionalized polymers is performed, for thiol-ene cross-linking, the allyl-ether-functionalized PGM is cross-linked with thiol-functionalized hyaluronic acid. Morphology, mechanical properties, and cell compatibility of both kinds of PGM hydrogels are characterized and compared. Furthermore, the biocompatibility of these hydrogels can be evaluated in cell culture experiments.…
Author: | Jessica S. Brand, Leonard Forster, Thomas Böck, Philipp Stahlhut, Jörg Teßmar, Jürgen Groll, Krystyna Albrecht |
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URN: | urn:nbn:de:bvb:20-opus-318453 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde |
Language: | English |
Parent Title (English): | Macromolecular Bioscience |
Year of Completion: | 2022 |
Volume: | 22 |
Issue: | 4 |
Article Number: | 2100274 |
Source: | Macromolecular Bioscience 2022, 22(4):2100274. DOI: 10.1002/mabi.202100274 |
DOI: | https://doi.org/10.1002/mabi.202100274 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | click chemistry; hydrogels; mucin; photopolymerization; thiol-ene |
Release Date: | 2023/07/06 |
Licence (German): | CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International |