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Biomimetic mineralization promotes viability and differentiation of human mesenchymal stem cells in a perfusion bioreactor
Please always quote using this URN: urn:nbn:de:bvb:20-opus-285804
- In bone tissue engineering, the design of 3D systems capable of recreating composition, architecture and micromechanical environment of the native extracellular matrix (ECM) is still a challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical stimuli, its use has been limited to a low number of biomaterials. In this work, we propose the culture of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to simultaneously provide biochemicalIn bone tissue engineering, the design of 3D systems capable of recreating composition, architecture and micromechanical environment of the native extracellular matrix (ECM) is still a challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical stimuli, its use has been limited to a low number of biomaterials. In this work, we propose the culture of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to simultaneously provide biochemical and biophysical cues guiding stem cell fate. The scaffolds were fabricated by mineralization of recombinant collagen in the presence of magnesium (RCP.MgAp). The organic matrix was homogeneously mineralized with apatite nanocrystals, similar in composition to those found in bone. X-Ray microtomography images revealed isotropic porous structure with optimum porosity for cell ingrowth. In fact, an optimal cell repopulation through the entire scaffolds was obtained after 1 day of dynamic seeding in the bioreactor. Remarkably, RCP.MgAp scaffolds exhibited higher cell viability and a clear trend of up-regulation of osteogenic genes than control (non-mineralized) scaffolds. Results demonstrate the potential of the combination of biomimetic mineralization of recombinant collagen in presence of magnesium and dynamic culture of hMSC as a promising strategy to closely mimic bone ECM.…
Author: | Gloria Belén Ramírez-Rodríguez, Ana Rita Pereira, Marietta Herrmann, Jan Hansmann, José Manuel Delgado-López, Simone Sprio, Anna Tampieri, Monica Sandri |
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URN: | urn:nbn:de:bvb:20-opus-285804 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Lehrstuhl für Orthopädie |
Language: | English |
Parent Title (English): | International Journal of Molecular Sciences |
ISSN: | 1422-0067 |
Year of Completion: | 2021 |
Volume: | 22 |
Issue: | 3 |
Article Number: | 1447 |
Source: | International Journal of Molecular Sciences 2021, 22(3), 1447; https://doi.org/10.3390/ijms22031447 |
DOI: | https://doi.org/10.3390/ijms22031447 |
Sonstige beteiligte Institutionen: | IZKF Nachwuchsgruppe Geweberegeneration für muskuloskelettale Erkrankungen |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | apatite nanoparticles; collagen; human mesenchymal stem cell; magnesium; osteogenesis; perfusion bioreactor; scaffold |
Release Date: | 2023/07/11 |
Date of first Publication: | 2021/02/01 |
EU-Project number / Contract (GA) number: | 607051 |
OpenAIRE: | OpenAIRE |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |