- search hit 1 of 1
The Folding Landscapes of Human Telomeric RNA and DNA G‐Quadruplexes are Markedly Different
Please always quote using this URN: urn:nbn:de:bvb:20-opus-238917
- We investigated the folding kinetics of G‐quadruplex (G4) structures by comparing the K\(^{+}\)‐induced folding of an RNA G4 derived from the human telomeric repeat‐containing RNA (TERRA25) with a sequence homologous DNA G4 (wtTel25) using CD spectroscopy and real‐time NMR spectroscopy. While DNA G4 folding is biphasic, reveals kinetic partitioning and involves kinetically favoured off‐pathway intermediates, RNA G4 folding is faster and monophasic. The differences in kinetics are correlated to the differences in the folded conformations of RNAWe investigated the folding kinetics of G‐quadruplex (G4) structures by comparing the K\(^{+}\)‐induced folding of an RNA G4 derived from the human telomeric repeat‐containing RNA (TERRA25) with a sequence homologous DNA G4 (wtTel25) using CD spectroscopy and real‐time NMR spectroscopy. While DNA G4 folding is biphasic, reveals kinetic partitioning and involves kinetically favoured off‐pathway intermediates, RNA G4 folding is faster and monophasic. The differences in kinetics are correlated to the differences in the folded conformations of RNA vs. DNA G4s, in particular with regard to the conformation around the glycosidic torsion angle χ that uniformly adopts anti conformations for RNA G4s and both, syn and anti conformation for DNA G4s. Modified DNA G4s with \(^{19}\)F bound to C2′ in arabino configuration adopt exclusively anti conformations for χ. These fluoro‐modified DNA (antiTel25) reveal faster folding kinetics and monomorphic conformations similar to RNA G4s, suggesting the correlation between folding kinetics and pathways with differences in χ angle preferences in DNA and RNA, respectively.…
Author: | Diana Müller, Irene Bessi, Christian Richter, Harald Schwalbe |
---|---|
URN: | urn:nbn:de:bvb:20-opus-238917 |
Document Type: | Journal article |
Faculties: | Fakultät für Chemie und Pharmazie / Institut für Organische Chemie |
Language: | English |
Parent Title (English): | Angewandte Chemie International Edition |
Year of Completion: | 2021 |
Volume: | 60 |
Issue: | 19 |
First Page: | 10895 |
Last Page: | 10901 |
Source: | Angewandte Chemie International Edition 2021, 60(19):10895–10901. DOI: 10.1002/anie.202100280 |
DOI: | https://doi.org/10.1002/anie.202100280 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Tag: | G-quadruplexes; TERRA RNA; folding landscapes; kinetics; real-time NMR spectroscopy |
Release Date: | 2021/12/20 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |