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Hypermethylated Chromosome Regions in Nine Fish Species with Heteromorphic Sex Chromosomes
Please always quote using this URN: urn:nbn:de:bvb:20-opus-196710
- Sites and amounts of 5-methylcytosine (5-MeC)-rich chromosome regions were detected in the karyotypes of 9 Brazilian species of Characiformes fishes by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. These species, belonging to the genera Leporinus, Triportheus and Hoplias, are characterized by highly differentiated and heteromorphic ZW and XY sex chromosomes. In all species, the hypermethylated regions are confined to constitutive heterochromatin. The number and chromosome locations of hypermethylated heterochromaticSites and amounts of 5-methylcytosine (5-MeC)-rich chromosome regions were detected in the karyotypes of 9 Brazilian species of Characiformes fishes by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. These species, belonging to the genera Leporinus, Triportheus and Hoplias, are characterized by highly differentiated and heteromorphic ZW and XY sex chromosomes. In all species, the hypermethylated regions are confined to constitutive heterochromatin. The number and chromosome locations of hypermethylated heterochromatic regions in the karyotypes are constant and species-specific. Generally, heterochromatic regions that are darkly stained by the C-banding technique are distinctly hypermethylated, but several of the brightly fluorescing hypermethylated regions merely exhibit moderate or faint C-banding. The ZW and XY sex chromosomes of all 9 analyzed species also show species-specific heterochromatin hypermethylation patterns. The analysis of 5-MeC-rich chromosome regions contributes valuable data for comparative cytogenetics of closely related species and highlights the dynamic process of differentiation operating in the repetitive DNA fraction of sex chromosomes.…
Author: | Michael Schmid, Claus Steinlein, Cassia F. Yano, Marcelo B. Cioffi |
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URN: | urn:nbn:de:bvb:20-opus-196710 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Humangenetik |
Language: | English |
Parent Title (English): | Cytogenetic and Genome Research |
ISSN: | 1424-8581 |
ISSN: | 1424-859X |
Year of Completion: | 2016 |
Volume: | 147 |
Issue: | 2-3 |
Pagenumber: | 169-178 |
Source: | Cytogenetic and Genome Research 2015;147(2-3):169–178. DOI: 10.1159/000444067 |
DOI: | https://doi.org/10.1159/000444067 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/26895457 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | 5-Methylcytosine; fish; heterochromatin; heteromorphic sex chromosomes; hypermethylation; immunofluorescence |
Release Date: | 2021/08/20 |
Date of first Publication: | 2016/02/20 |
Note: | This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively. |
Licence (German): | Deutsches Urheberrecht |