C. elegans Blastomeres Clear the Corpse of the Second Polar Body by LC3-Associated Phagocytosis
Please always quote using this URN: urn:nbn:de:bvb:20-opus-227651
- To understand how undifferentiated pluripotent cells cope with cell corpses, we examined the clearance of polar bodies born during female meiosis. We found that polar bodies lose membrane integrity and expose phosphatidylserine in Caenorhabditis elegans. Polar body signaling recruits engulfment receptors to the plasma membrane of embryonic blastomeres using the PI3K VPS-34, RAB-5 GTPase and the sorting nexin SNX-6. The second polar body is then phagocytosed using receptor-mediated engulfment pathways dependent on the Rac1 ortholog CED-10 butTo understand how undifferentiated pluripotent cells cope with cell corpses, we examined the clearance of polar bodies born during female meiosis. We found that polar bodies lose membrane integrity and expose phosphatidylserine in Caenorhabditis elegans. Polar body signaling recruits engulfment receptors to the plasma membrane of embryonic blastomeres using the PI3K VPS-34, RAB-5 GTPase and the sorting nexin SNX-6. The second polar body is then phagocytosed using receptor-mediated engulfment pathways dependent on the Rac1 ortholog CED-10 but undergoes non-apoptotic programmed cell death independent of engulfment. RAB-7 GTPase is required for lysosome recruitment to the polar body phagosome, while LC3 lipidation is required for degradation of the corpse membrane after lysosome fusion. The polar body phagolysosome vesiculates in an mTOR- and ARL-8-dependent manner, which assists its timely degradation. Thus, we established a genetic model to study clearance by LC3-associated phagocytosis and reveal insights into the mechanisms of phagosome maturation and degradation.…
Author: | Gholamreza Fazeli, Maurice Stetter, Jaime N. Lisack, Ann M. Wehman |
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URN: | urn:nbn:de:bvb:20-opus-227651 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Rudolf-Virchow-Zentrum |
Language: | English |
Parent Title (English): | Cell Reports |
Year of Completion: | 2018 |
Volume: | 23 |
Pagenumber: | 2070-2082 |
Source: | Cell Reports (2018) 23:2070-2082. https://doi.org/10.1016/j.celrep.2018.04.043 |
DOI: | https://doi.org/10.1016/j.celrep.2018.04.043 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Tag: | LC3-associated phagocytosis; cell corpse clearance; lysosomal degradation; non-apoptotic programmed cell death; non-canonical autophagy; phagolysosome tubulation; phagosome maturation; polar body |
Release Date: | 2024/08/08 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |