CD21\(^{lo/−}\)CD27\(^−\)IgM\(^−\) Double-Negative B Cells Accumulate in the Joints of Patients With Antinuclear Antibody-Positive Juvenile Idiopathic Arthritis
Please always quote using this URN: urn:nbn:de:bvb:20-opus-236286
- Juvenile idiopathic arthritis (JIA) encompasses a heterogeneous group of diseases. The appearance of antinuclear antibodies (ANAs) in almost half of the patients suggests B cell dysregulation as a distinct pathomechanism in these patients. Additionally, ANAs were considered potential biomarkers encompassing a clinically homogenous subgroup of JIA patients. However, in ANA+ JIA patients, the site of dysregulated B cell activation as well as the B cell subsets involved in this process is still unknown. Hence, in this cross-sectional study, weJuvenile idiopathic arthritis (JIA) encompasses a heterogeneous group of diseases. The appearance of antinuclear antibodies (ANAs) in almost half of the patients suggests B cell dysregulation as a distinct pathomechanism in these patients. Additionally, ANAs were considered potential biomarkers encompassing a clinically homogenous subgroup of JIA patients. However, in ANA+ JIA patients, the site of dysregulated B cell activation as well as the B cell subsets involved in this process is still unknown. Hence, in this cross-sectional study, we aimed in an explorative approach at characterizing potential divergences in B cell differentiation in ANA+ JIA patients by assessing the distribution of peripheral blood (PB) and synovial fluid (SF) B cell subpopulations using flow cytometry. The frequency of transitional as well as switched-memory B cells was higher in PB of JIA patients than in healthy controls. There were no differences in the distribution of B cell subsets between ANA- and ANA+ patients in PB. However, the composition of SF B cells was different between ANA- and ANA+ patients with increased frequencies of CD21\(^{lo/−}\)CD27\(^−\)IgM\(^−\) “double negative” (DN) B cells in the latter. DN B cells might be a characteristic subset expanding in the joints of ANA+ JIA patients and are potentially involved in the antinuclear immune response in these patients. The results of our explorative study might foster further research dissecting the pathogenesis of ANA+ JIA patients.…
| Author: | Johannes Dirks, Jonas Fischer, Gabriele Haase, Annette Holl-Wieden, Christine Hofmann, Hermann Girschick, Henner Morbach |
|---|---|
| URN: | urn:nbn:de:bvb:20-opus-236286 |
| Document Type: | Journal article |
| Faculties: | Medizinische Fakultät / Kinderklinik und Poliklinik |
| Language: | English |
| Parent Title (English): | Frontiers in Pediatrics |
| ISSN: | 2296-2360 |
| Year of Completion: | 2021 |
| Volume: | 9 |
| Article Number: | 635815 |
| Source: | Frontiers in Pediatrics (2021) 9:635815. doi: 10.3389/fped.2021.635815 |
| DOI: | https://doi.org/10.3389/fped.2021.635815 |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Tag: | B cells; antinuclear antibodies; double negative B cells; juvenile idiopathic arthritis; synovial fluid |
| Release Date: | 2022/02/04 |
| Date of first Publication: | 2021/04/16 |
| Open-Access-Publikationsfonds / Förderzeitraum 2021 | |
| Licence (German): |  CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |