Low energy status under methionine restriction is essentially independent of proliferation or cell contact inhibition
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-262329
- Nonlimited proliferation is one of the most striking features of neoplastic cells. The basis of cell division is the sufficient presence of mass (amino acids) and energy (ATP and NADH). A sophisticated intracellular network permanently measures the mass and energy levels. Thus, in vivo restrictions in the form of amino acid, protein, or caloric restrictions strongly affect absolute lifespan and age-associated diseases such as cancer. The induction of permanent low energy metabolism (LEM) is essential in this process. The murine cell line L929Nonlimited proliferation is one of the most striking features of neoplastic cells. The basis of cell division is the sufficient presence of mass (amino acids) and energy (ATP and NADH). A sophisticated intracellular network permanently measures the mass and energy levels. Thus, in vivo restrictions in the form of amino acid, protein, or caloric restrictions strongly affect absolute lifespan and age-associated diseases such as cancer. The induction of permanent low energy metabolism (LEM) is essential in this process. The murine cell line L929 responds to methionine restriction (MetR) for a short time period with LEM at the metabolic level defined by a characteristic fingerprint consisting of the molecules acetoacetate, creatine, spermidine, GSSG, UDP-glucose, pantothenate, and ATP. Here, we used mass spectrometry (LC/MS) to investigate the influence of proliferation and contact inhibition on the energy status of cells. Interestingly, the energy status was essentially independent of proliferation or contact inhibition. LC/MS analyses showed that in full medium, the cells maintain active and energetic metabolism for optional proliferation. In contrast, MetR induced LEM independently of proliferation or contact inhibition. These results are important for cell behaviour under MetR and for the optional application of restrictions in cancer therapy.…
Autor(en): | Corinna Koderer, Werner Schmitz, Anna Chiara Wünsch, Julia Balint, Mohamed El-Mesery, Julian Manuel Volland, Stefan Hartmann, Christian Linz, Alexander Christian Kübler, Axel Seher |
---|---|
URN: | urn:nbn:de:bvb:20-opus-262329 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Theodor-Boveri-Institut für Biowissenschaften |
Medizinische Fakultät / Klinik und Poliklinik für Mund-, Kiefer- und Plastische Gesichtschirurgie | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Cells |
ISSN: | 2073-4409 |
Erscheinungsjahr: | 2022 |
Band / Jahrgang: | 11 |
Heft / Ausgabe: | 3 |
Aufsatznummer: | 551 |
Originalveröffentlichung / Quelle: | Cells (2022) 11:3, 551. https://doi.org/10.3390/cells11030551 |
DOI: | https://doi.org/10.3390/cells11030551 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | L929; LC/MS; amino acid; caloric restriction; contact inhibition; liquid chromatography/mass spectrometry; mass spectrometry; metabolomics; methionine restriction; proliferation |
Datum der Freischaltung: | 03.02.2023 |
Datum der Erstveröffentlichung: | 04.02.2022 |
Open-Access-Publikationsfonds / Förderzeitraum 2022 | |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |