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Genome-wide transcriptome analysis of the plant pathogen Xanthomonas identifies sRNAs with putative virulence functions

Please always quote using this URN: urn:nbn:de:bvb:20-opus-131781
  • The Gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) is an important model to elucidate the mechanisms involved in the interaction with the host. To gain insight into the transcriptome of the Xcv strain 85-10, we took a differential RNA sequencing (dRNA-seq) approach. Using a novel method to automatically generate comprehensive transcription start site (TSS) maps we report 1421 putative TSSs in the Xcv genome. Genes in Xcv exhibit a poorly conserved -10 promoter element and no consensus Shine-DalgarnoThe Gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) is an important model to elucidate the mechanisms involved in the interaction with the host. To gain insight into the transcriptome of the Xcv strain 85-10, we took a differential RNA sequencing (dRNA-seq) approach. Using a novel method to automatically generate comprehensive transcription start site (TSS) maps we report 1421 putative TSSs in the Xcv genome. Genes in Xcv exhibit a poorly conserved -10 promoter element and no consensus Shine-Dalgarno sequence. Moreover, 14% of all mRNAs are leaderless and 13% of them have unusually long 5'-UTRs. Northern blot analyses confirmed 16 intergenic small RNAs and seven cis-encoded antisense RNAs in Xcv. Expression of eight intergenic transcripts was controlled by HrpG and HrpX, key regulators of the Xcv type III secretion system. More detailed characterization identified sX12 as a small RNA that controls virulence of Xcv by affecting the interaction of the pathogen and its host plants. The transcriptional landscape of Xcv is unexpectedly complex, featuring abundant antisense transcripts, alternative TSSs and clade-specific small RNAs.show moreshow less

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Metadaten
Author: Cornelius Schmidtke, Sven Findeiß, Cynthia M. Sharma, Juliane Kuhfuss, Steve Hoffmann, Jörg Vogel, Peter F. Stadler, Ulla Bonas
URN:urn:nbn:de:bvb:20-opus-131781
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Molekulare Infektionsbiologie
Language:English
Parent Title (English):Nucleic Acids Research
Year of Completion:2011
Volume:40
Issue:5
Source:Nucleic Acids Research, 2012, Vol. 40, No. 5, pp. 2020–2031. doi:10.1093/nar/gkr904
DOI:https://doi.org/10.1093/nar/gkr904
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:RNA; RNA sequence analyses; SUBSP carotovora; bacteria; campestris PV vesicatoria; consesus; determines pathgenicity; escherichia coli; extracellular enzymes; gene expression profiling; gene-cluster; genes; genetic transcription; genome; host (organism); hypersensitive response; libraries; maps; messanger RNA; northern blotting; pathogenetic organism; pathogenicity; plants; ralstonia solanacearum; regulatory RNA; secretion systems; small RNA; transcription initiation site; type III protein secretion system complex; type III secretion system pathways; untranslated regions; virulence; xanthomonas; xanthomonas campestris
Release Date:2016/12/20
First Page:2020
Last Page:2031
Licence (German):License LogoCC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell