Localization of PD‐L1 on single cancer cells by iSERS microscopy with Au/Au core/satellite nanoparticles
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- Programmed cell death‐ligand 1 (PD‐L1) is an important predictive biomarker. The detection of PD‐L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno‐SERS microscopy (iSERS) for localizing PD‐L1 on single cancer SkBr‐3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near‐infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotagsProgrammed cell death‐ligand 1 (PD‐L1) is an important predictive biomarker. The detection of PD‐L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno‐SERS microscopy (iSERS) for localizing PD‐L1 on single cancer SkBr‐3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near‐infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotags because of their remarkable signal brightness and colloidal stability upon red laser excitation. False‐color iSERS images of the positive and negative controls clearly reveal the specific localization of PD‐L1 with SERS nanotag‐labeled antibodies.…
Autor(en): | Elzbieta Stepula, Matthias König, Xin‐Ping Wang, Janina Levermann, Tobias Schimming, Sabine Kasimir‐Bauer, Bastian Schilling, Sebastian Schlücker |
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URN: | urn:nbn:de:bvb:20-opus-212655 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Klinik und Poliklinik für Dermatologie, Venerologie und Allergologie |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Journal of Biophotonics |
Erscheinungsjahr: | 2020 |
Band / Jahrgang: | 13 |
Heft / Ausgabe: | 3 |
Aufsatznummer: | e201960034 |
Originalveröffentlichung / Quelle: | Journal of Biophotonics 2020, 13(3):e201960034. DOI: 10.1002/jbio.201960034 |
DOI: | https://doi.org/10.1002/jbio.201960034 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | PD‐L1; Raman; SERS; gold nanoparticles |
Datum der Freischaltung: | 15.04.2021 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |