Single-cell RNA-seq: advances and future challenges
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-110993
- Phenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Singlecell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful tool to profile cell-to-cell variability on a genomic scale. Its application has already greatly impacted our conceptual understanding of diverse biological processes with broad implications for both basic and clinical research. DifferentPhenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Singlecell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful tool to profile cell-to-cell variability on a genomic scale. Its application has already greatly impacted our conceptual understanding of diverse biological processes with broad implications for both basic and clinical research. Different single-cell RNAseq protocols have been introduced and are reviewed here – each one with its own strengths and current limitations. We further provide an overview of the biological questions single-cell RNA-seq has been used to address, the major findings obtained from such studies, and current challenges and expected future developments in this booming field.…
Autor(en): | Stanislaw A. Gorski, Jörg Vogel, Antoine-Emmanuel Saliba, Alexander J. Westermann |
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URN: | urn:nbn:de:bvb:20-opus-110993 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Molekulare Infektionsbiologie |
Sprache der Veröffentlichung: | Englisch |
Erscheinungsjahr: | 2014 |
Originalveröffentlichung / Quelle: | Nucleic Acids Research, 2014, Vol. 42, No. 14, 8845–8860, doi: 10.1093/nar/gku555 |
DOI: | https://doi.org/10.1093/nar/gku555 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Normierte Schlagworte (GND): | RNS |
Datum der Freischaltung: | 18.03.2015 |
Sammlungen: | Open-Access-Publikationsfonds / Förderzeitraum 2014 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung |