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Non-Invasive Bioluminescence Imaging to Monitor the Immunological Control of a Plasmablastic Lymphoma-Like B Cell Neoplasia after Hematopoietic Cell Transplantation
Please always quote using this URN: urn:nbn:de:bvb:20-opus-111341
- To promote cancer research and to develop innovative therapies, refined pre-clinical mouse tumor models that mimic the actual disease in humans are of dire need. A number of neoplasms along the B cell lineage are commonly initiated by a translocation recombining c-myc with the immunoglobulin heavy-chain gene locus. The translocation is modeled in the C.129S1-Ighatm1(Myc)Janz/J mouse which has been previously engineered to express c-myc under the control of the endogenous IgH promoter. This transgenic mouse exhibits B cell hyperplasia andTo promote cancer research and to develop innovative therapies, refined pre-clinical mouse tumor models that mimic the actual disease in humans are of dire need. A number of neoplasms along the B cell lineage are commonly initiated by a translocation recombining c-myc with the immunoglobulin heavy-chain gene locus. The translocation is modeled in the C.129S1-Ighatm1(Myc)Janz/J mouse which has been previously engineered to express c-myc under the control of the endogenous IgH promoter. This transgenic mouse exhibits B cell hyperplasia and develops diverse B cell tumors. We have isolated tumor cells from the spleen of a C.129S1-Ighatm1(Myc)Janz/J mouse that spontaneously developed a plasmablastic lymphoma-like disease. These cells were cultured, transduced to express eGFP and firefly luciferase, and gave rise to a highly aggressive, transplantable B cell lymphoma cell line, termed IM380. This model bears several advantages over other models as it is genetically induced and mimics the translocation that is detectable in a number of human B cell lymphomas. The growth of the tumor cells, their dissemination, and response to treatment within immunocompetent hosts can be imaged non-invasively in vivo due to their expression of firefly luciferase. IM380 cells are radioresistant in vivo and mice with established tumors can be allogeneically transplanted to analyze graft-versus-tumor effects of transplanted T cells. Allogeneic hematopoietic stem cell transplantation of tumor-bearing mice results in prolonged survival. These traits make the IM380 model very valuable for the study of B cell lymphoma pathophysiology and for the development of innovative cancer therapies.…
Author: | Andreas Beilhack, Martin Chopra, Sabrina Kraus, Stefanie Schwinn, Miriam Ritz, Katharina Mattenheimer, Anja Mottok, Andreas Rosenwald, Hermann Einsele |
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URN: | urn:nbn:de:bvb:20-opus-111341 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Pathologisches Institut |
Medizinische Fakultät / Medizinische Klinik und Poliklinik II | |
Language: | English |
Year of Completion: | 2013 |
Source: | PLoS ONE 8(12): e81320. doi:10.1371/journal.pone.0081320 |
DOI: | https://doi.org/10.1371/journal.pone.0081320 |
Sonstige beteiligte Institutionen: | Center for Interdisciplinary Clinical Research, Würzburg University, Würzburg, Germany |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | B cells; Bioluminescence imaging; Bone marrow cells; Bone marrow transplantation; Cancer treatment; Lymphomas; Spleen; T cells |
Release Date: | 2015/04/02 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2014 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |