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Automated AFM analysis of DNA bending reveals initial lesion sensing strategies of DNA glycosylases
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-231338
- Base excision repair is the dominant DNA repair pathway of chemical modifications such as deamination, oxidation, or alkylation of DNA bases, which endanger genome integrity due to their high mutagenic potential. Detection and excision of these base lesions is achieved by DNA glycosylases. To investigate the remarkably high efficiency in target site search and recognition by these enzymes, we applied single molecule atomic force microscopy (AFM) imaging to a range of glycosylases with structurally different target lesions. Using a novel,Base excision repair is the dominant DNA repair pathway of chemical modifications such as deamination, oxidation, or alkylation of DNA bases, which endanger genome integrity due to their high mutagenic potential. Detection and excision of these base lesions is achieved by DNA glycosylases. To investigate the remarkably high efficiency in target site search and recognition by these enzymes, we applied single molecule atomic force microscopy (AFM) imaging to a range of glycosylases with structurally different target lesions. Using a novel, automated, unbiased, high-throughput analysis approach, we were able to resolve subtly different conformational states of these glycosylases during DNA lesion search. Our results lend support to a model of enhanced lesion search efficiency through initial lesion detection based on altered mechanical properties at lesions. Furthermore, its enhanced sensitivity and easy applicability also to other systems recommend our novel analysis tool for investigations of diverse, fundamental biological interactions.…
Autor(en): | Disha M. Bangalore, Hannah S. Heil, Christian F. Mehringer, Lisa Hirsch, Katharina Hemmen, Katrin G. Heinze, Ingrid Tessmer |
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URN: | urn:nbn:de:bvb:20-opus-231338 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Rudolf-Virchow-Zentrum |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Scientific Reports |
Erscheinungsjahr: | 2020 |
Band / Jahrgang: | 10 |
Aufsatznummer: | 15484 |
Originalveröffentlichung / Quelle: | Scientific Reports (2020) 10:15484. https://doi.org/10.1038/s41598-020-72102-7 |
DOI: | https://doi.org/10.1038/s41598-020-72102-7 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Freie Schlagwort(e): | 8-oxoguanine; atomic-force microscopy; base pairs; crystal structure; mismatches; molecular structure; recognition; repair; structural basis; thymine |
Datum der Freischaltung: | 21.04.2021 |
Open-Access-Publikationsfonds / Förderzeitraum 2020 | |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |