dRNA-Seq Reveals Genomewide TSSs and Noncoding RNAs of Plant Beneficial Rhizobacterium Bacillus amyloliquefaciens FZB42
Please always quote using this URN: urn:nbn:de:bvb:20-opus-138369
- Bacillus amyloliquefaciens subsp. plantarum FZB42 is a representative of Gram-positive plant-growth-promoting rhizobacteria (PGPR) that inhabit plant root environments. In order to better understand the molecular mechanisms of bacteria-plant symbiosis, we have systematically analyzed the primary transcriptome of strain FZB42 grown under rhizospheremimicking conditions using differential RNA sequencing (dRNA-seq). Our analysis revealed 4,877 transcription start sites for protein-coding genes, identified genes differentially expressed underBacillus amyloliquefaciens subsp. plantarum FZB42 is a representative of Gram-positive plant-growth-promoting rhizobacteria (PGPR) that inhabit plant root environments. In order to better understand the molecular mechanisms of bacteria-plant symbiosis, we have systematically analyzed the primary transcriptome of strain FZB42 grown under rhizospheremimicking conditions using differential RNA sequencing (dRNA-seq). Our analysis revealed 4,877 transcription start sites for protein-coding genes, identified genes differentially expressed under different growth conditions, and corrected many previously mis-annotated genes. We also identified a large number of riboswitches and cis-encoded antisense RNAs, as well as trans-encoded small noncoding RNAs that may play important roles in the gene regulation of Bacillus. Overall, our analyses provided a landscape of Bacillus primary transcriptome and improved the knowledge of rhizobacteria-host interactions.…
Author: | Ben Fan, Lei Li, Yanjie Chao, Konrad Förstner, Jörg Vogel, Rainer Borriss, Xiao-Qin Wu |
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URN: | urn:nbn:de:bvb:20-opus-138369 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Molekulare Infektionsbiologie |
Language: | English |
Parent Title (English): | PLoS One |
Year of Completion: | 2015 |
Volume: | 10 |
Issue: | 11 |
Pagenumber: | e0142002 |
Source: | PLoS ONE 10(11): e0142002. doi:10.1371/ journal.pone.0142002 |
DOI: | https://doi.org/10.1371/journal.pone.0142002 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | binding protein HFQ; enterica serovar thphimurium; escherichia coli; gene expression; listeria monocytogenes; messenger RNA; mycobacterium tuberculosis; small regulatory RNAs; subtilis genome; transcriptional landscape |
Release Date: | 2016/10/24 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |