The minimal meningococcal ProQ protein has an intrinsic capacity for structure-based global RNA recognition
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-230040
- FinO-domain proteins are a widespread family of bacterial RNA-binding proteins with regulatory functions. Their target spectrum ranges from a single RNA pair, in the case of plasmid-encoded FinO, to global RNA regulons, as with enterobacterial ProQ. To assess whether the FinO domain itself is intrinsically selective or promiscuous, we determine in vivo targets of Neisseria meningitidis, which consists of solely a FinO domain. UV-CLIP-seq identifies associations with 16 small non-coding sRNAs and 166 mRNAs. Meningococcal ProQ predominantly bindsFinO-domain proteins are a widespread family of bacterial RNA-binding proteins with regulatory functions. Their target spectrum ranges from a single RNA pair, in the case of plasmid-encoded FinO, to global RNA regulons, as with enterobacterial ProQ. To assess whether the FinO domain itself is intrinsically selective or promiscuous, we determine in vivo targets of Neisseria meningitidis, which consists of solely a FinO domain. UV-CLIP-seq identifies associations with 16 small non-coding sRNAs and 166 mRNAs. Meningococcal ProQ predominantly binds to highly structured regions and generally acts to stabilize its RNA targets. Loss of ProQ alters transcript levels of >250 genes, demonstrating that this minimal ProQ protein impacts gene expression globally. Phenotypic analyses indicate that ProQ promotes oxidative stress resistance and DNA damage repair. We conclude that FinO domain proteins recognize some abundant type of RNA shape and evolve RNA binding selectivity through acquisition of additional regions that constrain target recognition. FinO-domain proteins are bacterial RNA-binding proteins with a wide range of target specificities. Here, the authors employ UV CLIP-seq and show that minimal ProQ protein of Neisseria meningitidis binds to various small non-coding RNAs and mRNAs involved in virulence.…
Autor(en): | Saskia Bauriedl, Milan Gerovac, Nadja Heidrich, Thorsten Bischler, Lars Barquist, Jörg Vogel, Christoph Schoen |
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URN: | urn:nbn:de:bvb:20-opus-230040 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Hygiene und Mikrobiologie |
Medizinische Fakultät / Institut für Molekulare Infektionsbiologie | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Nature Communications |
Erscheinungsjahr: | 2020 |
Band / Jahrgang: | 11 |
Aufsatznummer: | 2823 |
Originalveröffentlichung / Quelle: | Nature Communications (2020) 11:2823. https://doi.org/10.1038/s41467-020-16650-6 | |
DOI: | https://doi.org/10.1038/s41467-020-16650-6 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | FinO family; HFQ; Neisseria meningitidis; chaperone; dual function; in vivo; natural transformation; regulator; sequence; transcriptome |
Datum der Freischaltung: | 19.04.2021 |
Sammlungen: | Open-Access-Publikationsfonds / Förderzeitraum 2020 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |