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Intravital imaging in spontaneously hypertensive stroke-prone rats-a pilot study

Please always quote using this URN: urn:nbn:de:bvb:20-opus-121353
  • Background There is growing evidence that endothelial failure and subsequent blood brain barrier (BBB) breakdown initiate cerebral small vessel disease (CSVD) pathology. In spontaneously hypertensive stroke-prone rats (SHRSP) endothelial damage is indicated by intraluminal accumulations of erythrocytes (erythrocyte thrombi) that are not observed with current magnetic resonance imaging techniques. Two-photon microscopy (2 PM) offers the potential for real-time direct detection of the small vasculature. Thus, within this pilot study weBackground There is growing evidence that endothelial failure and subsequent blood brain barrier (BBB) breakdown initiate cerebral small vessel disease (CSVD) pathology. In spontaneously hypertensive stroke-prone rats (SHRSP) endothelial damage is indicated by intraluminal accumulations of erythrocytes (erythrocyte thrombi) that are not observed with current magnetic resonance imaging techniques. Two-photon microscopy (2 PM) offers the potential for real-time direct detection of the small vasculature. Thus, within this pilot study we investigated the sensitivity of 2 PM to detect erythrocyte thrombi expressing initiating CSVD phenomena in vivo. Methods Eight SHRSP and 13 Wistar controls were used for in vivo imaging and subsequent histology with haematoxylin-eosin (HE). For 2 PM, cerebral blood vessels were labeled by fluorescent Dextran (70 kDa) applied intraorbitally. The correlation between vascular erythrocyte thrombi observed by 2 PM and HE-staining was assessed. Artificial surgical damage and parenchymal Dextran distribution were analyzed postmortem. Results Dextran was distributed within the small vessel walls and co-localized with IgG. Artificial surgical damage was comparable between SHRSP and Wistar controls and mainly affected the small vasculature. In fewer than 20% of animals there was correlation between erythrocyte thrombi as observed with 2 PM and histologically with HE. Conclusions Contrary to our initial expectations, there was little agreement between intravital 2 PM imaging and histology for the detection of erythrocyte thrombi. Two-photon microscopy is a valuable technique that complements but does not replace the value of conventional histology.show moreshow less

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Metadaten
Author: Solveig Niklass, Stoyan Stoyanov, Cornelia Garz, Celine Z. Bueche, Stine Mencl, Klaus Reymann, Hans-Jochen Heinze, Roxana O. Carare, Christoph Kleinschnitz, Stefanie Schreiber
URN:urn:nbn:de:bvb:20-opus-121353
Document Type:Journal article
Faculties:Medizinische Fakultät / Neurochirurgische Klinik und Poliklinik
Language:English
Parent Title (English):Experimental & Translational Stroke Medicine
Year of Completion:2014
Volume:6
Pagenumber:1
Source:Experimental & Translational Stroke Medicine 2014, 6:1. doi:10.1186/2040-7378-6-1
DOI:https://doi.org/10.1186/2040-7378-6-1
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/24461046
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:2 PM; CSVD; Intravital imaging; SHRSP
Release Date:2016/02/18
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung