Crystal Structure of a Variant PAM2 Motif of LARP4B Bound to the MLLE Domain of PABPC1
Please always quote using this URN: urn:nbn:de:bvb:20-opus-207800
- Eukaryotic cells determine the protein output of their genetic program by regulating mRNA transcription, localization, translation and turnover rates. This regulation is accomplished by an ensemble of RNA-binding proteins (RBPs) that bind to any given mRNA, thus forming mRNPs. Poly(A) binding proteins (PABPs) are prominent members of virtually all mRNPs that possess poly(A) tails. They serve as multifunctional scaffolds, allowing the recruitment of diverse factors containing a poly(A)-interacting motif (PAM) into mRNPs. We present the crystalEukaryotic cells determine the protein output of their genetic program by regulating mRNA transcription, localization, translation and turnover rates. This regulation is accomplished by an ensemble of RNA-binding proteins (RBPs) that bind to any given mRNA, thus forming mRNPs. Poly(A) binding proteins (PABPs) are prominent members of virtually all mRNPs that possess poly(A) tails. They serve as multifunctional scaffolds, allowing the recruitment of diverse factors containing a poly(A)-interacting motif (PAM) into mRNPs. We present the crystal structure of the variant PAM motif (termed PAM2w) in the N-terminal part of the positive translation factor LARP4B, which binds to the MLLE domain of the poly(A) binding protein C1 cytoplasmic 1 (PABPC1). The structural analysis, along with mutational studies in vitro and in vivo, uncovered a new mode of interaction between PAM2 motifs and MLLE domains.…
Author: | Clemens Grimm, Jann-Patrick Pelz, Cornelius Schneider, Katrin Schäffler, Utz Fischer |
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URN: | urn:nbn:de:bvb:20-opus-207800 |
Document Type: | Journal article |
Faculties: | Fakultät für Chemie und Pharmazie / Lehrstuhl für Biochemie |
Language: | English |
Parent Title (English): | Biomolecules |
ISSN: | 2218-273X |
Year of Completion: | 2020 |
Volume: | 10 |
Issue: | 6 |
Article Number: | 872 |
Source: | Biomolecules 2020, 10(6), 872; https://doi.org/10.3390/biom10060872 |
DOI: | https://doi.org/10.3390/biom10060872 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Tag: | MLLE domain; PABC1; PABP; PAM2; PAM2w; Poly(A) binding protein |
Release Date: | 2021/12/23 |
Date of first Publication: | 2020/06/06 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |