Identification and structural analysis of the Schizosaccharomyces pombe SMN complex
Please always quote using this URN: urn:nbn:de:bvb:20-opus-259880
- The macromolecular SMN complex facilitates the formation of Sm-class ribonucleoproteins involved in mRNA processing (UsnRNPs). While biochemical studies have revealed key activities of the SMN complex, its structural investigation is lagging behind. Here we report on the identification and structural determination of the SMN complex from the lower eukaryote Schizosaccharomyces pombe, consisting of SMN, Gemin2, 6, 7, 8 and Sm proteins. The core of the SMN complex is formed by several copies of SMN tethered through its C-terminal alpha-helicesThe macromolecular SMN complex facilitates the formation of Sm-class ribonucleoproteins involved in mRNA processing (UsnRNPs). While biochemical studies have revealed key activities of the SMN complex, its structural investigation is lagging behind. Here we report on the identification and structural determination of the SMN complex from the lower eukaryote Schizosaccharomyces pombe, consisting of SMN, Gemin2, 6, 7, 8 and Sm proteins. The core of the SMN complex is formed by several copies of SMN tethered through its C-terminal alpha-helices arranged with alternating polarity. This creates a central platform onto which Gemin8 binds and recruits Gemins 6 and 7. The N-terminal parts of the SMN molecules extrude via flexible linkers from the core and enable binding of Gemin2 and Sm proteins. Our data identify the SMN complex as a multivalent hub where Sm proteins are collected in its periphery to allow their joining with UsnRNA.…
Author: | Jyotishman VeepaschitORCiD, Aravindan ViswanathanORCiD, Remy BordonneORCiD, Clemens GrimmORCiD, Utz FischerORCiD |
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URN: | urn:nbn:de:bvb:20-opus-259880 |
Document Type: | Journal article |
Faculties: | Fakultät für Chemie und Pharmazie / Lehrstuhl für Biochemie |
Language: | English |
Parent Title (English): | Nucleic Acids Research |
Year of Completion: | 2021 |
Volume: | 49 |
Issue: | 13 |
Pagenumber: | 7207-7223 |
Source: | Nucleic Acids Research (2021) 49:13, 7207-7223. https://doi.org/10.1093/nar/gkab158 |
DOI: | https://doi.org/10.1093/nar/gkab158 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 572 Biochemie |
Tag: | SMN; Schizosaccharomyces pombe |
Release Date: | 2022/05/05 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2021 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |